AtWNK9 is regulated by ABA and dehydration and is involved in drought tolerance in Arabidopsis.
AtWNK9 is regulated by ABA and dehydration and is involved in drought tolerance in Arabidopsis.
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DOI：
10.1016/j.plaphy.2014.01.022
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发表时间：
2014-04
期刊：
影响因子：
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通讯作者：
Minmin Xie;Dan Wu;Guifang Duan;Liqun Wang;Reqing He;Xiushan Li;Dong-Ying Tang;Xiaoying Zhao
中科院分区：
文献类型：
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作者：
Minmin Xie;Dan Wu;Guifang Duan;Liqun Wang;Reqing He;Xiushan Li;Dong-Ying Tang;Xiaoying Zhao
WNK（withno lysine [K]）激酶在植物开花、耐作为盐和渗透胁迫等方面起着重要的调节作用。本文报道了拟南芥WNK基因家族成员AtWNK 9在外源脱落酸（阿坝）处理和脱水胁迫下的诱导表达。在拟南芥中过量表达花椰菜花叶病毒35 S启动子AtWNK 9，可提高拟南芥对阿坝的敏感性，强烈抑制初生根的伸长，增加脯氨酸的积累，减小气孔开度，降低失水速率。此外，与野生型相比，干旱胁迫下的植物存活得到改善。相比之下，AtWNK 9中插入T-DNA的突变体显示出阿坝敏感性降低和水分散失率增加;此外，它对干旱胁迫的敏感性也增加。阿坝诱导的转AtWNK 9基因植株中ABI 1、ERA 1、ABI 3和ABF 3等ABA信号转导元件的转录水平上调，而wnk 9突变体中ABI 1、ERA 1、ABI 3和ABF 3等ABA信号转导元件的转录水平下调。在脱水胁迫下，AtWNK 9转基因植株和wnk 9突变体中的一些ABA响应基因和生物合成基因以及其他干旱相关基因发生了不同程度的改变。总之，这些结果表明AtWNK 9在阿坝信号转导中起着积极的作用，并提高了转基因拟南芥的耐旱性。
WNK(withno lysine [K]) kinases play important regulatory roles in flowering, as well as salt and osmotic stress tolerance in plants. Here, we report thatAtWNK9, a member of the ArabidopsisWNKgene family, was induced by exogenous abscisic acid (ABA) treatment and dehydration stress. Overexpression ofAtWNK9from the cauliflower mosaic virus35Spromoter inArabidopsisresulted in increased sensitivity to ABA, strong inhibition of primary root elongation, increased proline accumulation, reduced stomatal aperture, and a reduced rate of water loss. In addition, plant survival under drought stress was improved compared to wild type. In contrast, a mutant with a T-DNA insertion inAtWNK9showed reduced ABA sensitivity and an increased rate of water loss; further, it showed increased susceptibility to drought stress. The transcription of a number of ABA signaling components, includingABI1,ERA1,ABI3, andABF3, was up-regulated inAtWNK9transgenic plants and down-regulated in thewnk9mutant in response to ABA. Some ABA-responsive and biosynthetic genes, as well as other drought-related genes, were altered at various levels inAtWNK9transgenic plants andwnk9mutants under dehydration stress. Overall, these findings suggest thatAtWNK9plays a positive role in ABA signaling and improves drought tolerance in transgenicArabidopsis.