DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L.
DNA polymorphisms in the tetrahydrocannabinolic acid (THCA) synthase gene in "drug-type" and "fiber-type" Cannabis sativa L.
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DOI：
10.1016/j.forsciint.2005.07.005
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发表时间：
2006-06-02
影响因子：
2.2
通讯作者：
Muranaka, Toshiya
中科院分区：
文献类型：
--
作者：
Kojoma, Mareshige;Seki, Hikaru;Muranaka, Toshiya
对13个不同品系的大麻（Cannabis sativa L.）进行了分析6株属于“药物型”类，具有高Δ-9-四氢大麻酚酸（THCA）含量，7株属于“纤维型”类，使用HPLC分析具有低THCA。研究了来自每个菌株的THCA合酶基因的基因组DNA序列多态性。从6株“药物型”植物中检测到THCA合酶基因的单一PCR片段。我们也可以检测到片段从7株“纤维型”植物，虽然没有或非常低的含量THCA检测在这些样品中。这13个菌株共扩增出1638个内含子，其中没有内含子。在“毒品型”和“纤维型”大麻植物中分别存在THCA合酶基因的两种变体。从这些变体的推导的氨基酸序列的比对中检测到37个主要的取代。此外，我们确定了一个特定的PCR标记的THCA合酶基因的“药物型”菌株。在“纤维型”菌株中未检测到该PCR标记。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
The cannabinoid content of 13 different strains of cannabis plant (Cannabis sativa L.) was analyzed. Six strains fell into the "drug-type" class, with high Delta-9-tetrahydrocannabinolic acid (THCA) content, and seven strains into the "fiber-type" class, with low THCA using HPLC analysis. Genomic DNA sequence polymorphisms in the THCA synthase gene from each strain were studied. A single PCR fragment of the THCA synthase gene was detected from six strains of "drug-type" plants. We could also detect the fragment from seven strains of "fiber-type" plants, although no or very low content of THCA were detected in these samples. These were 1638 by from all 13 strains and no intron among the sequences obtained. There were two variants of the THCA synthase gene in the "drug-type" and "fiber-type" cannabis plants, respectively. Thirty-seven major substitutions were detected in the alignment of the deduced amino acid sequences from these variants. Furthermore, we identified a specific PCR marker for the THCA synthase gene for the "drug-type" strains. This PCR marker was not detected in the "fiber-type" strains. (c) 2005 Elsevier Ireland Ltd. All rights reserved.