Investigation of an LC-MS-MS (QTrap®) method for the rapid screening and identification of drugs in postmortem toxicology whole blood samples
Investigation of an LC-MS-MS (QTrap®) method for the rapid screening and identification of drugs in postmortem toxicology whole blood samples
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DOI：
10.1093/jat/29.7.599
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发表时间：
2005-10-01
影响因子：
2.5
通讯作者：
Wall, WH
中科院分区：
文献类型：
--
作者：
Herrin, GL;McCurdy, HH;Wall, WH
应用生物系统公司的QTrap®液相色谱-串联质谱仪(LC-MS-MS)作为酶免疫分析(EIA)的辅助手段，用于快速检测血液中的药物。因此，用EIA鉴定全血中药物的方法被扩展到LC-MS-MS分析。建立了一个包含100多种药物的多反应监测(MRM)数据库，对死后毒理学案例中常见的药物进行分析。QTrap方法提供的扫描时间仅为2.8%S，以产生MRM和增强产品离子扫描。给出了该方法的各种验证和开发步骤，以及作为最终验证手段的一致性研究。本研究比较了QTrap、常规提取方法和气相色谱-质谱法在95份尸检样品中鉴定毒品的有效性。这项研究中的400多个药物结果显示，这两种技术之间100%一致。
The QTrap®liquid chromatography-tandem mass spectrometry (LC-MS-MS) by Applied Biosystems was investigated as an adjunct to enzyme immunoassay (EIA) for the rapid detection of drugs in blood. Thus, a procedure used to identify drugs in whole blood by EIA was extended to LC-MS-MS analysis. A multiple reaction monitoring (MRM) database of over 100 drugs was constructed to analyze for those drugs commonly observed in postmortem toxicology cases. The QTrap method provided for a scan time of only 2.8 s to produce both an MRM and an enhanced product ion scan. Various validation and developmental steps of the method are presented, as well as a concordance study as a final means of validation. This study was conducted to compare the effectiveness of the QTrap versus conventional extraction methods and gas chromatography-MS for the identification of drugs in 95 postmortem samples. The more than 400 drug results in this study showed 100% concordance between the two techniques.