Detection and characterization of an emerging type of Babesia sp. similar to Babesia motasi for the first case of human babesiosis and ticks in Korea
Detection and characterization of an emerging type of Babesia sp. similar to Babesia motasi for the first case of human babesiosis and ticks in Korea
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DOI：
10.1080/22221751.2019.1622997
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发表时间：
2019-06-09
影响因子：
13.2
通讯作者：
Lee, Sang-Eun
中科院分区：
文献类型：
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作者：
Hong, Sung-Hee;Kim, Seong-Yoon;Lee, Sang-Eun
巴贝虫病是一种蜱传播的红细胞内人畜共患病。在韩国，2005年报告了由于在绵羊中检测到巴贝虫而导致的第一例死亡;在此，我们报告了第二例巴贝虫病的流行病学和遗传特征。患者血液的显微镜分析显示多态性裂殖子。为了检测巴氏杆菌属，使用巴氏杆菌特异性引物对β-微管蛋白、18 S rDNA、COB和COX 3基因片段进行PCR。18 S rDNA分析，与绵羊巴氏杆菌属和2005年韩国巴氏杆菌感染的同源性为98%。此外，18 S rDNA，CO B和COX 3的系统发育分析显示与B的剂量相关。motasi。为了鉴定传染源，在巴贝虫病患者的先前居住地周围收集了长角血蜱（296）和特拉瓦血蜱（301）。在3个H.长角类：鉴定为B类。microti和两个样品与B的同源性为98%。motasi。我们的研究是第一次直接确认人类巴贝虫病的传染源。该病例最有可能是由巴贝斯虫病患者房屋附近的蜱虫叮咬引起的。H. longicornis被认为是B的载体。microti和其他Babysp.感染。
Babesiosis is a tick-transmitted intraerythrocytic zoonosis. In Korea, the first mortalities were reported in 2005 due to Babesia sp. detection in sheep; herein we report epidemiological and genetic characteristics of a second case of babesiosis. Microscopic analysis of patient blood revealed polymorphic merozoites. To detect Babesia spp., PCR was performed using Babesia specific primers for beta-tubulin, 18S rDNA, COB, and COX3 gene fragments. 18S rDNA analysis for Babesia sp., showed 98% homology with ovine Babesia sp. and with Babesia infections in Korea in 2005. Moreover, phylogenetic analysis of 18S rDNA, COB, and COX3 revealed dose associations with B. motasi. For identifying the infectious agent, Haemaphysalis longicornis (296) and Haemaphysalis tlava (301) were collected around the previous residence of the babesiosis patient. Babesia genes were identified in three H. longicornis: one sample was identified as B. microti and two samples were 98% homologous to B. motasi. Our study is the first direct confirmation of the infectious agent for human babesiosis. This case most likely resulted from tick bites from ticks near the patient house of the babesiosis patient. H. longicornis has been implicated as a vector of B. microti and other Babesia sp. infections.