The Triticum ispahanicum elongated glume locus P2 maps to chromosome 6A and is associated with the ectopic expression of SVP-A1.
The Triticum ispahanicum elongated glume locus P2 maps to chromosome 6A and is associated with the ectopic expression of SVP-A1.
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DOI：
10.1007/s00122-022-04114-y
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发表时间：
2022-07
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我们提出的MADS盒转录因子SVP-A1作为一个有前途的候选基因延长颖座P2，它映射到染色体6A，而不是以前提出的染色体7 B。在水稻和小麦中，颖壳和花器官长度与籽粒大小呈正相关，使其成为增加籽粒大小和潜在产量的重要目标。已知小麦亚种Triticum ispahanicum发育伸长的颖片和花器官以及长的谷粒。这些多重表型效应是由P2基因座控制的，该基因座先前定位于小麦染色体7 B。使用三个映射人口，我们表明，长颖座P2不映射到染色体7 B，而是映射到染色体6A上的1.68 Mbp的间隔。在这段时间内，我们鉴定了SVP-A1，一种MADS盒转录因子，它是玉米基因的直接直系同源物，该基因是“豆荚玉米”被囊动物基因座的基础，并且是T. polonicum elongated glume P1基因。于T. ispahanicum中，我们确定了一个独特的等位基因，该等位基因在SVP-A1启动子中具有482-bp缺失，并且与SVP-A1在伸长颖片和花器官中的异位和更高表达相关。我们使用近等基因系（NILs）表明，P2对颖片，外稃，内稃，穗和籽粒的长度有一致的正效应。根据定位数据、自然变异、SVP基因的生物学功能和表达分析，我们提出MADS盒转录因子SVP-A1是P2的一个有希望的候选基因。在线版本包含补充材料，可通过10.1007/s 00122 -022-04114-y获得。
We propose the MADS-box transcription factor SVP-A1 as a promising candidate gene for the elongated glume locus P2, which maps to chromosome 6A instead of the previously proposed chromosome 7B. In rice and wheat, glume and floral organ length are positively correlated with grain size, making them an important target to increase grain size and potentially yield. The wheat subspecies Triticum ispahanicum is known to develop elongated glumes and floral organs as well as long grains. These multiple phenotypic effects are controlled by the P2 locus, which was previously mapped to wheat chromosome 7B. Using three mapping populations, we show that the long glume locus P2 does not map to chromosome 7B, but instead maps to a 1.68 Mbp interval on chromosome 6A. Within this interval, we identified SVP-A1, a MADS box transcription factor which is the direct ortholog of the maize gene underlying the ‘pod corn’ Tunicate locus and is a paralog to the T. polonicum elongated glume P1 gene. In T. ispahanicum, we identified a unique allele which has a 482-bp deletion in the SVP-A1 promoter and is associated with ectopic and higher expression of SVP-A1 in the elongated glumes and floral organs. We used near-isogenic lines (NILs) to show that P2 has a consistent positive effect on the length of glume, lemma, palea, spike and grain. Based on the mapping data, natural variation, biological function of SVP genes in cereals and expression analyses, we propose the MADS-box transcription factor SVP-A1 as a promising candidate for P2. The online version contains supplementary material available at 10.1007/s00122-022-04114-y.