Chemical Studies on Tobacco Smoke LXVII. Quantitative Determination of Alkaloids in Tobacco by Liquid Chromatography
Chemical Studies on Tobacco Smoke LXVII. Quantitative Determination of Alkaloids in Tobacco by Liquid Chromatography
复制标题
烟草烟雾的化学研究 LXVII。
DOI：
--
复制
发表时间：
1980
期刊：
影响因子：
--
通讯作者：
D. Hoffmann
中科院分区：
文献类型：
--
作者：
J. Piadé;D. Hoffmann
摘要 建立了一种定量测定烟草提取物基本组分中各个生物碱的高效液相色谱法。使用两个反相 RP-18 柱，用三乙胺磷酸盐缓冲液水溶液 (pH 7.56) 和乙腈梯度洗脱。该方法经过优化，在时间因素、灵敏度和准确性方面与现有定量技术相比具有优势。
Abstract An HPLC method was developed for the quantitative determination of individual alkaloids of the basic fraction of tobacco extracts. Two reverse phase RP-18 columns were used, eluting with a gradient of aqueous triethylamine phosphate buffer (pH 7.56) and acetonitrile. The method was optimized and compared favorably with existing quantitative techniques in regard to time factors, sensitivity and accuracy.