Optimization of aqueous enzymatic method for Camellia sinensis oil extraction and reuse of enzymes in the process
Optimization of aqueous enzymatic method for Camellia sinensis oil extraction and reuse of enzymes in the process
复制标题
水酶法提取茶油的工艺优化及酶的重复利用
DOI：
10.1016/j.jbiosc.2019.05.010
复制
发表时间：
2019
影响因子：
2.8
通讯作者：
孟祥河
中科院分区：
文献类型：
--
作者：
彭丽;叶沁;刘晓颖;刘书来;孟祥河
对水相酶法提取茶油进行了研究。结果表明，超声辅助脱皂素预处理能有效地减少乳化，提高游离油回收率。用70%异丙醇超声提取30min后，再用游离纤维素酶和碱性酶进一步水解5h，同时加入钙离子(Nca 2D：nSapin[1：2])，可得到94.14%的游离油回收率。采用海藻酸盐包埋和戊二醛交联法分别对碱性酶和纤维素酶进行固定化和共固定化。碱性酶和纤维素酶的活力和活力恢复都是可以接受的。固定化后的碱性酶和纤维素酶在较宽的pH和温度范围内表现出较高的活力。进行了固定化酶的重复使用实验。模拟了固定化酶的失活动力学，估算了固定化酶的半衰期。结果表明，磁性载体有利于从茶籽泥中回收固定化酶，固定化碱性酶和纤维素酶具有良好的重复使用性。
Aqueous enzymatic extraction of Camellia sinensis oil was studied. The results suggested that saponin removal pretreatment assisted by ultrasound was effective in decreasing emulsification and in enhancing the free oil recovery. After.70% isopropanol extraction for 30 min under ultrasound, the residue of C. sinensis seeds was further hydrolyzed with.free cellulase and Alcalase for 5 h, and calcium ions were concurrently added during enzymatic hydrolysis.(nCa 2D: nsaponin [ 1:2), and free oil recovery up to 94.14% was obtained. Separate immobilization and co-immobilization of.Alcalase and cellulase were performed by alginate entrapment combined with glutaraldehyde crosslinking. Specific.activity and recovery of activity for Alcalase and cellulase were acceptable. After immobilization, Alcalase and cellulase.exhibited higher activity at a wider pH and temperature range. Reuse experiments of immobilized enzymes were.conducted. The deactivation kinetics immobilized enzymes were simulated and half-life of immobilized enzyme was.estimated. The results indicated that a magnetic supporter facilitated the recovery of immobilized enzymes from tea.seed slurry, and that immobilized Alcalase and cellulase had good reusability.