Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells
Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells
复制标题
DOI：
10.1016/j.bbrc.2007.06.023
复制
发表时间：
2007-08-24
影响因子：
3.1
通讯作者：
Yokochi, Takashi
中科院分区：
文献类型：
--
作者：
Islam, Shamima;Hassan, Ferdaus;Yokochi, Takashi
脂多糖(LPS)是一种强有力的骨吸收因子。以小鼠RAW 264.7巨噬细胞为模型，观察内毒素对破骨细胞形成的影响。染毒3天后，脂多糖可诱导RAW 264.7细胞形成多核巨细胞。NIGCs抗酒石酸酸性磷酸酶(TRAP)活性阳性。此外，MGC在作为破骨细胞底物的钙磷薄膜上形成了吸收坑。因此，建议用脂多糖诱导RAW 264.7细胞形成破骨细胞。抗肿瘤坏死因子-α抗体、巨噬细胞集落刺激因子抗体和核因子受体激活剂-kappaB配体抗体均可阻断脂多糖诱导的破骨细胞形成。肿瘤坏死因子-α可能在脂多糖诱导的RAW 264.7细胞破骨细胞形成中起重要作用。核因子-kappaB和应激激活蛋白激酶(SAPK/JNK)的抑制剂可抑制内毒素诱导的破骨细胞的形成。对内毒素诱导破骨细胞形成的详细机制进行了讨论。(C)2007 Elsevier Inc.保留所有权利。
Lipopolysaccharide (LPS) is a potent bone resorbing factor. The effect of LPS on osteoclast formation was examined by using murine RAW 264.7 macrophage cells. LPS-induced the formation of multinucleated giant cells (MGC) in RAW 264.7 cells 3 days after the exposure. NIGCs were positive for tartrate-resistant acid phosphatase (TRAP) activity. Further, MGC formed resorption pits on calcium-phosphate thin film that is a substrate for osteoclasts. Therefore, LPS was suggested to induce osteoclast formation in RAW 264.7 cells. LPS-induced osteoclast formation was abolished by anti-tumor necrosis factor (TNF)-alpha antibody, but not antibodies to macrophage-colony stimulating factor (M-CSF) and receptor activator of nuclear factor (NF)-kappa B ligand (RANKL). TNF-alpha might play a critical role in LPS-induced osteoclast formation in RAW 264.7 cells. Inhibitors of NF-kappa B and stress activated protein kinase (SAPK/JNK) prevented the LPS-induced osteoclast formation. The detailed mechanism of LPS-induced osteoclast formation is discussed. (C) 2007 Elsevier Inc. All rights reserved.