A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines
A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines
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牛副流感病毒 3 型融合蛋白中的单个 L288I 取代可增强病毒在半合适细胞系中的生长
DOI：
10.1007/s00705-017-3378-1
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发表时间：
2017
期刊：
影响因子：
2.7
通讯作者：
Takeuchi Kaoru
中科院分区：
文献类型：
--
作者：
Matsuura Ryosuke;Takada Marina;Kokuho Takehiro;Tsuboi Takamitsu;Kameyama Ken-ichiro;Takeuchi Kaoru
用牛副流感病毒1株在鸡胚成纤维细胞（CEF）中连续传代，获得了牛副流感病毒3型BN-CE疫苗株。我们之前在两个菌株之间的融合(F)蛋白中发现了一个取代（L288I）。为了研究替代对CEF适应和衰减的影响，我们在F蛋白中替换了L288I (FL288I-EGFP)，构建了重组BN-1菌株。FL288I-EGFP在半合适细胞系中的复制效率高于重组BN-1菌株（wt-EGFP），表明在适应CEF的过程中，BN-1菌株建立了L288I替代。
The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (FL288I-EGFP). FL288I-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.