The influence of chronic nicotine treatment on proteins expressed in the mouse hippocampus and cortex
The influence of chronic nicotine treatment on proteins expressed in the mouse hippocampus and cortex
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DOI：
10.1016/j.ejphar.2016.03.025
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发表时间：
2016-06-05
影响因子：
5
通讯作者：
Matsuyama, Shogo
中科院分区：
文献类型：
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作者：
Matsuura, Kenji;Otani, Mieko;Matsuyama, Shogo
尼古丁（烟草烟雾中的主要精神活性物质）的长期治疗会影响中枢神经系统功能，例如突触可塑性。在这里，为了阐明长期尼古丁治疗对高级大脑功能的影响，使用二维凝胶电泳 (2-DE) 和质谱分析对接受尼古丁治疗 6 个月的小鼠的海马体和皮质进行蛋白质组学分析。海马 16 种蛋白和 1 种磷蛋白的表达发生显着变化（微管蛋白 β-5、atp5b、MDH1、细胞色素 b-c1 复合体亚基 1、Hsc70、dynamin、profilin-2、4-氨基丁酸转氨酶、线粒体异构体 1 前体、钙蛋白酶小亚基 1 和液泡腺苷三磷酸酶亚基增加） B 和减少的 γ-肌动蛋白、α-微管蛋白同种型 M-α-2、推定的 β-肌动蛋白、微管蛋白 β-2A、NDUFA10 和 G6PD）以及皮质中的 24 种蛋白质和两种磷蛋白（增加的血影蛋白 α 链、非红细胞 1 亚型 1、微管蛋白 β-5、γ-肌动蛋白、肌酸激酶 B 型、LDH-B、 Secernin-1、UCH-L1、14-3-3 γ、II 型过氧化还原蛋白 1、PEBP-1 和未命名蛋白产物以及减少的微管蛋白 α-1C、α-internexin、γ-烯醇酶、PDHE1-B、DPYL2、空泡三磷酸腺苷酶亚基 A、空泡三磷酸腺苷酶亚基 B、TCTP、 NADH 脱氢酶 Fe-S 蛋白 1、蛋白二硫键异构酶 A3、hnRNP H2、γ-肌动蛋白、atp5b 和未命名的蛋白产物）。此外，蛋白质印迹验证了 dynamin、Hsc70、MDH1、NDUFA10、α-internexin、微管蛋白 beta-5 链和 secernin-1 的变化。因此，这些发现表明，长期尼古丁治疗改变了海马和皮质中蛋白质和磷蛋白的表达。我们认为吸烟对高级大脑功能的影响可能是通过改变这些蛋白质的表达水平来介导的。 (C) 2016 年由 Elsevier B.V. 出版
Chronic treatment with nicotine, the primary psychoactive substance in tobacco smoke, affects central nervous system functions, such as synaptic plasticity. Here, to clarify the effects of chronic nicotine treatment on the higher brain functions, proteomic analysis of the hippocampus and cortex of mice treated for 6 months with nicotine was performed using two-dimensional gel electrophoresis (2-DE) followed by mass spectrometry. There was significant change in the expression of 16 proteins and one phosphoprotein in the hippocampus (increased tubulin beta-5, atp5b, MDH1, cytochrome b-c1 complex subunit 1, Hsc70, dynamin, profilin-2, 4-aminobutyrate aminotransferase, mitochondrial isoform 1 precursor, calpain small subunit 1, and vacuolar adenosine triphosphatase subunit B and decreased gamma-actin, alpha-tubulin isotype M-alpha-2, putative beta-actin, tubulin beta-2A, NDUFA10, and G6PD) and 24 proteins and two phosphoproteins in the cortex (increased spectrin alpha chain, non-erythrocytic 1 isoform 1, tubulin beta-5, gamma-actin, creatine kinase B-type, LDH-B, secernin-1, UCH-L1, 14-3-3 gamma, type II peroxiredoxin 1, PEBP-1, and unnamed protein product and decreased tubulin alpha-1C, alpha-internexin, gamma-enolase, PDHEl-B, DPYL2, vacuolar adenosine triphosphatase subunit A, vacuolar adenosine triphosphatase subunit B, TCTP, NADH dehydrogenase Fe-S protein 1, protein disulfide-isomerase A3, hnRNP H2, gamma-actin, atp5b, and unnamed protein product). Additionally, Western blotting validated the changes in dynamin, Hsc70, MDH1, NDUFA10, alpha-internexin, tubulin beta-5 chain, and secernin-1. Thus, these findings indicate that chronic nicotine treatment changes the expression of proteins and phosphoproteins in the hippocampus and cortex. We propose that effect of smoking on higher brain functions could be mediated by alterations in expression levels of these proteins. (C) 2016 Published by Elsevier B.V.