CLUSTERS OF COUPLED NEUROBLASTS IN EMBRYONIC NEOCORTEX
CLUSTERS OF COUPLED NEUROBLASTS IN EMBRYONIC NEOCORTEX
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DOI：
10.1126/science.1850552
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发表时间：
1991-04-26
期刊：
影响因子：
56.9
通讯作者：
KRIEGSTEIN, AR
中科院分区：
文献类型：
--
作者：
LOTURCO, JJ;KRIEGSTEIN, AR
大脑的新皮层从简单的皮层发展成复杂的多层结构。 为了研究早期新皮层发育过程中的细胞相互作用，采用全细胞膜片钳技术记录了胎鼠脑室区的成神经细胞。 在早期皮质生成过程中，神经母细胞通过缝隙连接生理性地偶联成15至90个细胞的簇。 偶联的细胞在心室区形成柱状，并且由于它们在簇中的成员资格，具有低表观膜电阻，并且对抑制性神经递质γ-氨基丁酸产生大的反应。 随着神经元迁移出脑室区的进展，簇内的细胞数量减少。 这些簇允许在皮质生成的最早阶段直接细胞与细胞的相互作用。
The neocortex of the brain develops from a simple germinal layer into a complex multilayer structure. To investigate cellular interactions during early neocortical development, whole-cell patch clamp recordings were made from neuroblasts in the ventricular zone of fetal rats. During early corticogenesis, neuroblasts are physiologically coupled by gap junctions into clusters of 15 to 90 cells. The coupled cells form columns within the ventricular zone and, by virtue of their membership in clusters, have low apparent membrane resistances and generate large responses to the inhibitory neurotransmitter gamma-aminobutyric acid. As neuronal migration out of the ventricular zone progresses, the number of cells within the clusters decreases. These clusters allow direct cell to cell interaction at the earliest stages of corticogenesis.