Diagnosis of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry.
Diagnosis of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry.
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使用 PCR-电喷雾电离质谱法诊断假体关节感染。
DOI：
10.1128/jcm.03217-13
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发表时间：
2014
影响因子：
9.4
通讯作者：
Patel,Robin
中科院分区：
文献类型：
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作者：
Greenwood-Quaintance,KerrylE;Uhl,JamesR;Hanssen,ArlenD;Sampath,Rangarajan;Mandrekar,JayawantN;Patel,Robin
我们比较了431例膝关节移位(270例)或髋关节假体(161例)受试者的聚合酶链式反应-电喷雾电离/质谱仪(PCR-ESI/MS)和超声液培养。在这些受试者中，152人和279人分别患有假体关节感染(PJI)和无菌失败。聚合酶链式反应-电喷雾电泳法和培养法检测肺炎链球菌感染的敏感性分别为77.6%和69.7%(P=0.0105)。特异性分别为93.5%和99.3%(P=0.0002)。
We compared PCR-electrospray ionization mass spectrometry (PCR-ESI/MS) to culture using sonicate fluid from 431 subjects with explanted knee (n= 270) or hip (n= 161) prostheses. Of these, 152 and 279 subjects had prosthetic joint infection (PJI) and aseptic failure, respectively. The sensitivities for detecting PJI were 77.6% for PCR-ESI/MS and 69.7% for culture (P= 0.0105). The specificities were 93.5 and 99.3%, respectively (P= 0.0002).