Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines.
Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines.
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使用染色体片段替代系绘制水稻花期耐热性数量性状位点
DOI：
10.1270/jsbbs.15084
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发表时间：
2016-06
期刊：
影响因子：
2.4
通讯作者：
Yan S
中科院分区：
文献类型：
--
作者：
Zhao L;Lei J;Huang Y;Zhu S;Chen H;Huang R;Peng Z;Tu Q;Shen X;Yan S
为了研究开花期耐热性的遗传基础，以粳稻Sasanishiki为材料，利用染色体片段代换系（CSSLs）对开花期耐热性进行了研究。热敏感）和Habataki（indica spp.）耐热）用于在三种高温环境下进行分析。测定了高温条件下小麦的小穗育性、日开花时间和花粉脱落量。检测到11个相关QTL，其中2个与小穗育性相关的QTL qSFht2和qSFht4. 2分别位于第2和第4染色体上。在第3、8、10和11染色体上检测到4个影响日开花时间的QTL qDFT3、qDFT8、qDFT10. 1和qDFT11。另外5个QTL qPSLht1、qPSLht4.1、qPSLht5、qPSLht7和qPSLht10.2分别位于第1、4、5、7和10染色体上，对小穗育性和花粉脱落水平均有影响。在这11个QTL中，有8个与已报道的QTL重叠，其中3个QTL qPSLht4.1、qPSLht7和qPSLht10.2为新QTL。进一步验证了qPSLht4. 1基因在不同温度下的稳定性，可用于提高花粉在柱头上的脱落和生长，为水稻耐热育种提供参考。
To study the genetic basis of heat tolerance at anthesis, a set of chromosome segment substitution lines (CSSLs) derived from Sasanishiki (japonica ssp. heat susceptible) and Habataki (indica spp. heat tolerant) were used for analysis across three high temperature environments. Spikelet fertility (SF), daily flowering time (DFT) and pollen shedding level (PSL) under high temperature (HT) were assessed. Eleven related QTLs were detected, of which, two QTLs qSFht2 and qSFht4.2 for spikelet fertility were identified on chromosomes 2 and 4. Four QTLs qDFT3, qDFT8, qDFT10.1 and qDFT11 for daily flowering time were detected on chromosomes 3, 8, 10 and 11. The other five QTLs qPSLht1, qPSLht4.1, qPSLht5, qPSLht7 and qPSLht10.2 on chromosomes 1, 4, 5, 7 and 10, respectively, were found had effects both on spikelet fertility and pollen shedding level. Of the 11 QTLs, 8 were overlapped with QTLs reported by others, 3 QTLs qPSLht4.1, qPSLht7 and qPSLht10.2 identified in this study were novel. The stability of qPSLht4.1 was further verified at different temperatures, which could be used to improve the pollen shedding and pollen growth on stigma for rice heat-tolerance breeding.