Colonic mucin composition in primates. Selective alterations associated with spontaneous colitis in the cotton-top tamarin.
Colonic mucin composition in primates. Selective alterations associated with spontaneous colitis in the cotton-top tamarin.
复制标题
灵长类动物的结肠粘蛋白成分。
DOI：
10.1016/s0016-5085(85)80127-x
复制
发表时间：
1985
期刊：
影响因子：
29.4
通讯作者：
Winter,HS
中科院分区：
文献类型：
--
作者：
Podolsky,DK;Madara,JL;King,N;Sehgal,P;Moore,R;Winter,HS
在不同灵长类动物的直肠粘膜活检标本中检测到结肠粘蛋白糖蛋白Wa_1的异质性(Saguinus Oedipus，n=18；猕猴，n=2；Saca f ascularis，n=2；Aotus trivirgatus，n=2；Saimiri scureus，n=2；Callithrix Jacchus，n=2)。在初步分离放射性标记的粘蛋白和从粘膜活检标本中溶解的非粘蛋白糖蛋白后，在猴的直肠粘膜中发现了至少5种标记的粘蛋白成分，而在人的结肠中观察到了6种粘蛋白组分。虽然灵长类动物一直缺乏人类结肠粘蛋白中存在的最早的洗脱成分，但其他粘蛋白成分与先前在人类结肠活检标本中发现的可比组分进行了共层析。在所有物种中，每个组分的相对比例都是一致的，除了会患慢性结肠炎的棉顶罗兰(S.Oedipus)。研究发现，棉顶粉中一种粘蛋白成分(IV)的数量显著减少，其方式类似于先前在溃疡性结肠炎患者中发现的人类粘蛋白成分的减少。在一项为期10周的双盲交叉研究中，对柳氮磺胺吡啶(50 mg/kg·d)或安慰剂处理的12个棉顶罗望子的粘蛋白谱进行了连续评估，表明与疾病活动性无关的第四种罗望子中的选择性减少是持续的。相反，治疗后与疾病活动性增加相关的他马林粘蛋白III的相对量大于与疾病活动性降低相关的粘蛋白III的相对量(46%±11%对19%±7%)。
Heterogeneity of colonic mucin glycoprotein wa,, examined in rectal mucosal biopsy specimens frorr a variety of primate species (Saguinus oedipus, n = 18; Macaca mulatta, n = 2; Macaca f ascicularis, n = 2; Aotus trivirgatus, n = 2; Saimiri sciureus, n = 2; and Callithrix jacchus, n = 2). After initial separation of radiolabeled mucin and nonmucin glycoproteins solubilized from mucosal biopsy specimens, al least five labeled mucin components were found in monkey rectal mucosa in contrast to the six mucin fractions observed in the human colon. Although primates consistently lacked the earliest eluting component present in human colonic mucin, other mucin components cochromatographed with comparable fractions previously identified in human colonic biopsy specimens. The relative proportions of each fraction were consistent throughout all species except the cotton-top tamarin (S. oedipus), an animal that develops a chronic colitis. The cottontop tamarin was found to have a markedly reduced amount of one mucin component (IV) in a manner analogous to the reduction in a human .mucin fraction previously noted in patients with ulcerative colitis. Sequential evaluation of mucin profiles in cotton-top tamarins (n = 12) treated with sulfasalazine (50 mg/kg · day) or placebo in a 10-wk doubleblind crossover study demonstrated the persistence of the selective reduction in tamarin species IV unrelated to disease activity. In contrast, the relative amount of tamarin mucin III was greater in association with increased disease activity than that observed in association with reduced disease activity (46% ± 11% total mucin vs. 19% ± 7% total mucin posttreatment).