Effects of local immunization with glucosyltransferase fractions from Streptococcus mutans on dental caries in rats and hamsters.
Effects of local immunization with glucosyltransferase fractions from Streptococcus mutans on dental caries in rats and hamsters.
复制标题
变形链球菌葡萄糖基转移酶组分局部免疫对大鼠和仓鼠龋齿的影响。
DOI：
10.4049/jimmunol.118.2.710
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发表时间：
1977
影响因子：
4.4
通讯作者：
D. Smith
中科院分区：
文献类型：
--
作者：
M. Taubman;D. Smith
研究了变形链球菌葡萄糖基转移酶局部免疫对常规大鼠、仓鼠和非生物大鼠龋齿的影响。给这些动物注射含有完全弗氏佐剂的粗糖基转移酶或已定义的葡萄糖基转移酶制剂，结果一致导致唾液中存在抗体，这可以通过酶活性的功能性抑制和放射性酶的结合来证明。血清抗体也存在。在常规大鼠和非生物大鼠中完成了两项实验，其中动物用粗酶抗原免疫。免疫组动物的平均龋齿评分始终低于假免疫或未免疫对照组。用含有不超过三种抗原成分（其中两种是酶）的特定酶制剂免疫的仓鼠，也显示出平均龋齿评分显著降低。免疫动物的病变数量也总是较低。在某些情况下，可以从免疫感染动物的牙齿中恢复的变形链球菌数量减少。光滑的牙表面比咬合表面的龋齿和病变的减少更大，这可能是变形链球菌所表现出的粘附现象的干扰。提出抗体干扰影响由该菌引起的龋病。
The effect of local immunization with glucosyltransferase enzymes of Streptococcus mutans on dental caries in conventional rats, hamsters, and gnotobiotic rats was studied. Injection of these animals with crude or defined glucosyltransferase enzyme preparations incorporated into complete Freund's adjuvant consistently resulted in the presence of antibody in saliva demonstrable by functional inhibition of enzymatic activity and binding of radioactive enzyme. Serum antibody was also present. Two experiments have been completed in conventional rats and one in gnotobiotic rats in which the animals were immunized with crude enzyme antigens. The immunized groups of animals always had lower mean caries scores than comparably sham-immunized or nonimmunized control groups. Hamsters immunized with a defined enzyme preparation, containing no more than three antigenic components (two of which were enzyme), also demonstrated significant reductions in mean caries scores. The numbers of lesions were also always lower in immunized animals. In some cases there were reductions in the numbers of S. mutans that could be recovered from the teeth of immunized, infected animals. The reductions in dental caries and lesions were greater on smooth dental surfaces than on occlusal surfaces, which might be explained as interference with adherence phenomena demonstrated by S. mutans. It is proposed that antibody interference affects dental caries caused by this organism.