A recently identified ovine Babesia in China: Serology and sero-epidemiology
A recently identified ovine Babesia in China: Serology and sero-epidemiology
复制标题
最近在中国发现的羊巴贝虫病：血清学和血清流行病学
DOI：
10.1016/j.parint.2012.04.004
复制
发表时间：
2012-12-01
影响因子：
1.9
通讯作者：
Luo, Jianxun
中科院分区：
文献类型：
--
作者：
Guan, Guiquan;Ma, Miling;Luo, Jianxun
新疆巴贝虫是由璃眼蜱传播的一种大型巴贝虫，对小型反刍动物具有感染性，但对健康绵羊几乎没有致病性。根据18SrRNA和ITS基因序列、形态特征、媒介蜱种和致病性，将其鉴定为一个新的蜱种。本研究以新疆Babyrosp. in Xinjiang（BXJMA）裂殖子体外培养的可溶性抗原为抗原，建立了酶联免疫吸附试验（ELISA）。以特异性平均抗体率的24.65%为阳性阈值，特异性和敏感性均为97.3%。ELISA和Western blot检测结果表明，BXJMA与其它绵羊感染梨形虫和绵羊无形体病羊的阳性血清无交叉反应。实验感染后2周即可检测到新疆巴氏杆菌特异性抗体，且高水平抗体可持续12周以上。用ELISA法检测了22个省、市、自治区50个地（市）的3857份小反刍动物血清，平均阳性率为31.66%。这些数据表明，所开发的ELISA是新疆巴贝虫血清学诊断的有力工具，并证实该虫是一个新物种。(C)2012爱思唯尔爱尔兰有限公司保留所有权利。
Babesia sp. in Xinjiang, transmitted by Hyalomma, is a large Babesia that is infective for small ruminants, but it has almost no pathogenicity in healthy sheep. On the basis of the sequences of the 18S rRNA and internal transcribed spacer (ITS) genes, morphological characteristics, vector tick species and pathogenicity it was identified recently as a novel Babesia species. In the present study, an enzyme-linked immunosorbent assay (ELISA) was developed using soluble merozoite antigens of Babesia sp. in Xinjiang (BXJMA) derived from in vitro culture. When the positive threshold was chosen as 24.65% of the specific mean antibody rate, the specificity and sensitivity were both 97.3%. There was no cross-reaction between BXJMA and positive sera from sheep infected with other Chinese ovine piroplasms or Anaplasma ovis in the ELISA and western blotting. Specific antibodies against Babesia sp. in Xinjiang could be detected 2 weeks post infection and a high level of antibodies persisted for more than 12 weeks in experimentally infected sheep. The ELISA was tested on 3857 sera collected from small ruminants in 50 prefectures of 22 provinces to evaluate the sero-epidemiology of Babesia sp. in Xinjiang infection, and the average positive rate was 31.66%. These data provide that the developed ELISA is a powerful tool for the sero-diagnosis of Babesia sp. in Xinjiang and confirm that it is a novel species. (C) 2012 Elsevier Ireland Ltd. All rights reserved.