Development of a poly(dimethylsiloxane) interface for on-line capillary column liquid chromatography-capillary electrophoresis coupled to sheathless electrospray ionization time-of-flight mass spectrometry
Development of a poly(dimethylsiloxane) interface for on-line capillary column liquid chromatography-capillary electrophoresis coupled to sheathless electrospray ionization time-of-flight mass spectrometry
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DOI：
10.1021/ac030117g
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发表时间：
2003-10-15
影响因子：
7.4
通讯作者：
Markides, KE
中科院分区：
文献类型：
--
作者：
Bergström, SK;Samskog, J;Markides, KE
本文研究了填充毛细管液相色谱（LC）在线正交耦合的弹性聚二甲基硅氧烷（PDMS）界面。= 0.2 mm）的毛细管电泳（CE）结合无鞘电喷雾电离（ESI）飞行时间质谱（TOFMS）检测。新接口具有两级设计，其与通过接口的连续CE电解质流相结合，提供LC流出物和CE分离的完整性，直到需要注入。当与熔融石英柱技术结合时，发现透明和柔性PDMS材料具有许多优点，包括易于遵循该过程和易于更换柱。通过分别结合LC、CE和ESI-MS的常规微尺度系统，协调各个维度的时间尺度以获得每单位时间的最佳峰容量。以多肽为模型物质，对LC-CE-TOFMS系统的性能进行了评价。从25 μ g/mL肽标准品的0.5穆尔LC进样中获得约330的亮氨酸脑啡肽S/N。
An interface in elastomeric poly(dimethylsiloxane) (PDMS) for on-line orthogonal coupling of packed capillary liquid chromatography (LC) (i.d. = 0.2 mm) with capillary electrophoresis (CE) in combination with sheathless electrospray ionization (ESI) time-of-flight mass spectrometric (TOFMS) detection is presented. The new interface has a two-level design, which in combination with a continuous CE electrolyte flow through the interface provides integrity of the LC effluent and the CE separation until an injection is desired. The transparent and flexible PDMS material was found to have a number of advantages when combined with fused silica column technology, including ease to follow the process and ease to exchange columns. By combining conventional microscale systems of LC, CE, and ESI-MS, respectively, the time scales of the individual dimensions were harmonized for optimal peak capacity per unit time. The performance of the LC-CE-TOFMS system was evaluated using peptides as model substances. A S/N of about 330 was achieved for leucineenkephaline from a 0.5 muL LC injection of 25 mug/mL peptide standard.