Regulation of CAD gene expression in mouse fibroblasts during the transition from the resting to the growing state.
Regulation of CAD gene expression in mouse fibroblasts during the transition from the resting to the growing state.
复制标题
小鼠成纤维细胞从静息状态向生长状态转变期间 CAD 基因表达的调节。
DOI：
10.1016/0014-4827(88)90413-2
复制
发表时间：
1988
影响因子：
3.7
通讯作者：
Church,RL
中科院分区：
文献类型：
--
作者：
Rao,GN;Church,RL
我们分析了 BALB/c 3T3 小鼠成纤维细胞在静止状态和血清刺激开始后不同时间点的 CAD mRNA 和 ATCase 活性的稳态水平。血清刺激 12 小时后，3T3 细胞中 CAD mRNA 的稳态水平比静止时测量的水平增加了 10 倍。与观察到的 CAD mRNA 稳态水平的增加相反，其转录率仅增加了 3 倍，这表明这些细胞中 CAD 基因的表达在转录和转录后水平上受到调节，在很大程度上受到后者的调节。血清刺激细胞中 CAD mRNA 的增加伴随着 ATCase 活性的平行增加。当将 DNA 合成（[3H]胸苷摄取）与 CAD mRNA 和 ATCase 活性的积累进行比较时，观察到这种积累发生在细胞周期的中后期 G1 期。这些结果表明CAD基因的表达是细胞生长依赖性的并且可能是DNA合成的先决条件。
We have analyzed the steady-state levels of CAD mRNA and ATCase activity in BALB/c 3T3 mouse fibroblasts at quiescence and at various time points following the initiation of serum stimulation. Steady-state levels of CAD mRNA in 3T3 cells following 12 h of serum stimulation increased 10-fold over levels measured at quiescence. In contrast to the observed increase in steady-state levels of CAD mRNA, its rate of transcription increased only 3-fold, suggesting that the expression of CAD gene in these cells is regulated at both the transcriptional and post-transcriptional levels, to a major extent by the latter. These increases in CAD mRNA in serum-stimulated cells were followed by parallel increases in ATCase activity as well. When comparing DNA synthesis ([3H]thymidine uptake) to the accumulation of CAD mRNA and ATCase activity, it was observed that this accumulation occurred during the mid- to late-G1phase of the cell cycle. These results suggest that the expression of CAD gene is cell growth dependent and may be a prerequisite to DNA synthesis.