Evolution of Streptococcus pneumoniae Serotype 3 in England and Wales: A Major Vaccine Evader
Evolution of Streptococcus pneumoniae Serotype 3 in England and Wales: A Major Vaccine Evader
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DOI：
10.3390/genes10110845
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发表时间：
2019-11-01
期刊：
影响因子：
3.5
通讯作者：
Fry, Norman K.
中科院分区：
文献类型：
--
作者：
Groves, Natalie;Sheppard, Carmen L.;Fry, Norman K.
尽管肺炎链球菌结合疫苗13（PCV 13）中包含了肺炎链球菌血清型3，但它仍然是英格兰和威尔士侵袭性肺炎球菌疾病的主要原因。以往的研究表明，血清型3克隆复合物180内存在不同的谱系，近年来随着进化枝II的出现，进化枝分布发生了变化。我们对2003年至2018年期间来自英格兰和威尔士的616种血清型3分离株进行了全基因组测序和基因组分析，包括侵袭性和携带分离株。我们的调查显示，自2014年以来，进化枝II已经扩大，现在代表了英格兰和威尔士50%的血清型3侵袭性肺炎球菌病（IPD）分离株。抗生素抗性和蛋白抗原基因的基因组分析表明，不同的配置文件中存在的分支，这可以解释最近出现的这个分支。这项研究强调了常规全基因组测序的重要性和实用性，以及它在单核苷酸水平上识别新的和正在出现的变异的能力，这为监测提供了信息，并将影响未来的疫苗开发。
Despite its inclusion in pneumococcal conjugate vaccine 13 (PCV13), Streptococcus pneumoniae serotype 3 remains a major cause of invasive pneumococcal disease in England and Wales. Previous studies have indicated that there are distinct lineages within serotype 3 clonal complex 180 and the clade distributions have shifted in recent years with the emergence of clade II. We undertook whole genome sequencing and genomic analysis of 616 serotype 3 isolates from England and Wales between 2003 and 2018, including invasive and carriage isolates. Our investigations showed that clade II has expanded since 2014 and now represents 50% of serotype 3 invasive pneumococcal disease (IPD) isolates in England and Wales. Genomic analysis of antibiotic resistance and protein antigen genes showed that distinct profiles are present within the clades which could account for the recent emergence of this clade. This investigation highlights the importance and utility of routine whole genome sequencing and its ability to identify new and emerging variation at the single nucleotide level which informs surveillance and will impact future vaccine development.