Overexpression of PHRF1 attenuates the proliferation and tumorigenicity of non-small cell lung cancer cells.
Overexpression of PHRF1 attenuates the proliferation and tumorigenicity of non-small cell lung cancer cells.
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PHRF1 的过度表达减弱非小细胞肺癌细胞的增殖和致瘤性
DOI：
10.18632/oncotarget.11842
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发表时间：
2016-09-27
期刊：
影响因子：
--
通讯作者：
Yang H
中科院分区：
文献类型：
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作者：
Wang Y;Wang H;Pan T;Li L;Li J;Yang H
本研究的目的是探讨PHRF 1在肺肿瘤发生中的潜在作用。Western blot分析蛋白表达。采用定量逆转录聚合酶链反应、免疫组化、软琼脂法和裸鼠成瘤实验。流式细胞仪分析细胞周期分布。肺癌组织中PHRF 1 mRNA表达水平低于癌旁组织。H1299和H1650细胞系中PHRF 1蛋白的表达较16 HBE和BEAS-2B细胞系中PHRF 1蛋白的表达降低。PHRF 1蛋白在恶性16 HBE细胞中的表达较对照细胞降低。与对照组相比，BaP处理组小鼠肺组织PHRF 1蛋白表达明显降低。过表达PHRF 1可抑制H1299细胞的增殖、集落形成和体内移植瘤的生长，并使细胞周期阻滞于G1期。与H1299-pvoid细胞相比，H1299-PHRF 1细胞中TGIF和c-Myc蛋白表达降低，p21蛋白表达升高。总之，我们的研究结果表明，PHRF 1的过表达减弱了非小细胞肺癌细胞系H1299的增殖和致瘤性。
The aim of this study was to investigate the potential role of PHRF1 in lung tumorigenesis. Western blot analysis was used to detect the expression of proteins. Quantitative reverse transcriptase polymerase chain reaction, immunohistochemistry, soft agar assay and tumor formation assay in nude mice were applied. Cell cycle distribution was analyzed by flow cytometry. The lower level of PHRF1 mRNA was observed in human lung cancer tissues than that in paracancerous tissues. The decreased expression of PHRF1 protein was observed in H1299 and H1650 cell lines than that in 16HBE and BEAS-2B cell lines. The decreased expression of PHRF1 protein was observed in malignant 16HBE cells compared to control cells. The reduced expression of PHRF1 protein was observed in mice lung tissues treated with BaP than that in control group. Overexpression of PHRF1 inhibited H1299 cell proliferation, colony formation in vitro and growth of tumor xenograft in vivo, and arrested cell cycle in G1 phase. The decreased expression of TGIF and c-Myc proteins and the increased expression of p21 protein were observed in H1299-PHRF1 cells compared with H1299-pvoid cells. In conclusion, our findings suggest that overexpression of PHRF1 attenuated the proliferation and tumorigenicity of non-small cell lung cancer cell line of H1299.