A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains.
A Mr 46,000 human milk fat globule protein that is highly expressed in human breast tumors contains factor VIII-like domains.
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发表时间：
1991-09
期刊：
影响因子：
11.2
通讯作者：
David Larocca;Jerry A. Peterson;R. Urrea;Jon Kuniyoshi;A. Bistrain;Roberto L. Ceriani
中科院分区：
文献类型：
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作者：
David Larocca;Jerry A. Peterson;R. Urrea;Jon Kuniyoshi;A. Bistrain;Roberto L. Ceriani
人乳脂肪球已被证明是生产针对乳腺上皮细胞表面成分的抗体的良好抗原材料来源。针对人乳脂球主要成分之一的单克隆抗体鉴定出表观分子量为46,000的糖蛋白，已被发现对乳腺癌的诊断和治疗都很有用。为了鉴定这种Mr 46,000糖蛋白，我们使用特异性单克隆抗体从哺乳期乳腺的lambda gt11表达文库中选择互补dna。最大的互补DNA插入片段BA46-1有1270个碱基对，编码217个氨基酸。利用这种互补DNA探针，在多种癌细胞系中特异性检测到一个2.2千碱基的RNA，并在一些癌细胞系中过表达。Western blot检测mRNA表达水平与抗原表达水平相关。序列分析显示Mr 46,000糖蛋白与血清因子VIII和V在涉及磷脂结合的区域具有很强的同源性。
The human milk fat globule has proved to be a good source of antigenic material for production of antibodies against surface components of breast epithelial cells. Monoclonal antibodies against one of the major components of the human milk fat globule, which identify a glycoprotein with an apparent molecular weight of 46,000, have been found to be useful for both breast cancer diagnosis and therapy. In order to characterize this Mr 46,000 glycoprotein, specific monoclonal antibodies were used to select complementary DNAs from a lambda gt11 expression library from lactating breast. The largest complementary DNA insert (BA46-1) was 1270 base pairs and encoded 217 amino acids. A single 2.2-kilobase RNA was specifically detected in a variety of carcinoma cell lines, using this complementary DNA probe, and it was overexpressed in some carcinoma lines. The mRNA levels correlated with the level of expression of the antigen in these cell lines as detected by Western blot analysis. Sequence analysis revealed strong homology of the Mr 46,000 glycoprotein with serum factors VIII and V, in the region implicated in phospholipid binding.