A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana.
A chemical genetic strategy identify the PHOSTIN, a synthetic molecule that triggers phosphate starvation responses in Arabidopsis thaliana.
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DOI：
10.1111/nph.13591
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
Desnos T
中科院分区：
文献类型：
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作者：
Bonnot C;Pinson B;Clément M;Bernillon S;Chiarenza S;Kanno S;Kobayashi N;Delannoy E;Nakanishi TM;Nussaume L;Desnos T
植物表现出多种应对磷酸盐（Pi）缺乏的策略。尽管有多种遗传学研究，低Pi信号的分子机制仍不清楚。为了验证化学遗传学对研究这一途径的兴趣，我们发现并分析了PHOSTIN （PSN）的作用，这是一种在拟南芥中模拟Pi -饥饿的药物。我们评估了PSN及其结构类似物在突变体和野生型中诱导π缺乏症反应的作用，并通过高压液相色谱法（HPLC）或质谱法跟踪了它们在植物器官中的积累。我们发现PSN在生长培养基中被劈裂，释放其活性基序（PSN11），并在植物根系中积累。尽管在处理过的植株根系中存在π的过度积累，但PSN11引发了局部和全身的π饥饿效应。然而，尽管PSN11对低Pi基因的转录激活在phr1中丢失；PHO1双突变体，PHO1和PHO2都不需要PSN11效应。Pi -饥饿引发的局部和全身反应的范围及其对PHR1/PHL1功能的依赖表明，PSN11影响Pi -饥饿信号传导的重要和早期步骤。它与PHO1和PHO2的独立表明存在未知途径，显示PSN和化学遗传学为该领域带来新元素的有用性。
Plants display numerous strategies to cope with phosphate (Pi)‐deficiency. Despite multiple genetic studies, the molecular mechanisms of low‐Pi‐signalling remain unknown. To validate the interest of chemical genetics to investigate this pathway we discovered and analysed the effects of PHOSTIN (PSN), a drug mimicking Pi‐starvation in Arabidopsis. We assessed the effects of PSN and structural analogues on the induction of Pi‐deficiency responses in mutants and wild‐type and followed their accumulation in plants organs by high pressure liquid chromotography (HPLC) or mass‐spectrophotometry. We show that PSN is cleaved in the growth medium, releasing its active motif (PSN11), which accumulates in plants roots. Despite the overaccumulation of Pi in the roots of treated plants, PSN11 elicits both local and systemic Pi‐starvation effects. Nevertheless, albeit that the transcriptional activation of low‐Pi genes by PSN11 is lost in the phr1;phl1 double mutant, neither PHO1 nor PHO2 are required for PSN11 effects. The range of local and systemic responses to Pi‐starvation elicited, and their dependence on the PHR1/PHL1 function suggests that PSN11 affects an important and early step of Pi‐starvation signalling. Its independence from PHO1 and PHO2 suggest the existence of unknown pathway(s), showing the usefulness of PSN and chemical genetics to bring new elements to this field.