Inhibitory effects of a chemically standardized extract from Scutellaria barbata in human colon cancer cell lines, LoVo
Inhibitory effects of a chemically standardized extract from Scutellaria barbata in human colon cancer cell lines, LoVo
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DOI：
10.1021/jf051506
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发表时间：
2005-10-19
影响因子：
6.1
通讯作者：
Ong, ES
中科院分区：
文献类型：
--
作者：
Goh, D;Lee, YH;Ong, ES
半枝莲（SB）是一种药用植物，含有黄酮类化合物，如灯盏乙素、灯盏乙素、红花素、异红花素和汉黄芩素。采用功能蛋白质组学方法研究SB的化学标准化提取物对人结肠肾上腺癌LoVo的抑制作用。在这项工作中，稳定的同位素没有用于所提出的方法开发。用胰蛋白酶消化来自对照和处理细胞的全细胞裂解物，并通过二维（阳离子交换和反相）液相色谱和串联质谱法分离肽。差异表达的蛋白质鉴定使用目前的方法支持从细胞周期分析与流式细胞术获得的数据。用流式细胞术分析，用更高剂量的SIB提取物观察到亚G期的显著增加。我们的研究结果表明，化学标准化提取物从SIB可以诱导细胞死亡的人结肠癌细胞系。我们目前的工作表明，所提出的平台提供了一种快速的方法来研究分子机制，因为不同剂量的植物提取物对LoVo细胞系的抑制作用。这包括参与代谢、细胞周期调节和转录因子活性的蛋白质网络。
Scutellaria barbata (SB) is a medicinal plant that contains flavonone compounds such as scutellarein, scutellarin, carthamidin, isocarthamidin, and wogonin. A functional proteomic approach was used to study the inhibitory effects of a chemically standardized extract from SB in human colon adrencarcinoma, LoVo. In this work, a stable isotope was not used in the proposed method developed. The whole cell lysates from the control and treated cells were digested with trypsin, and the peptides were separated by two-dimensional (cation-exchange and reversed-phase) liquid chromatography and tandem mass spectrometry. The differentially expressed proteins identified using the current approach supported the data obtained from cell-cycle analysis with flow cytometry. With flow-cytometry analysis, a significant increase in the sub G, phase was observed with a higher dose of extract from SIB. Our results suggest that the chemically standardized extract from SIB can induce cell death in the human colon cancer cell line. Our current work showed that the proposed platform provided a rapid approach to study the molecular mechanism because of the inhibitory effects of different doses of the botanical extracts on LoVo cell lines. This included a network of proteins involved in metabolism, regulation of the cell cycle, and transcription-factor activity.