Rapid in vitro ectomycorrhizal infection on Pinus densiflora roots by Tricholoma matsutake
Rapid in vitro ectomycorrhizal infection on Pinus densiflora roots by Tricholoma matsutake
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DOI：
10.1007/bf02463952
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发表时间：
2000-08-15
期刊：
影响因子：
1.4
通讯作者：
Suzuki, Kazuo
中科院分区：
文献类型：
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作者：
Guerin-Laguette, Alexis;Vaario, Lu-Min;Suzuki, Kazuo
用松口蘑菌丝悬浮液接种11周龄赤松幼苗的根系，并在不提供外源碳水化合物的森林土壤中无菌培养4周。接种后一周，真菌菌丝已定殖根表面和绑定土壤颗粒在一起，建立一个根基质连续。真菌菌丝是可见的主根皮层后，清除，漂白和染色。在随后的日子里，真菌定植观察到延长侧根，其中哈蒂格网形成确认接种后4周。这是首次报道赤松幼苗被T.松茸。
The root systems of 11-wk-old Pinus densiflora seedlings were inoculated with a hyphal suspension of Tricholoma matsutake and aseptically incubated for 4 wk in a forest soil without supplying exogenous carbohydrates. One week following inoculation, fungal hyphae had colonized the root surface and bound soil particles together establishing a root-substrate continuum. Fungal hyphae were visible within the main root cortex following clearing, bleaching and staining. In the ensuing days, fungal colonization was observed within elongating lateral roots in which Hartig net formation was confirmed 4 wk after inoculation. This is the first report of rapid ectomycorrhizal infection of P. densiflora seedlings by T. matsutake.