Heterotopic Heart Transplantation in Rats: Improved Anesthetic and Surgical Technique
Heterotopic Heart Transplantation in Rats: Improved Anesthetic and Surgical Technique
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DOI：
10.1016/j.transproceed.2010.07.097
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发表时间：
2010-11-01
影响因子：
0.9
通讯作者：
Trento, A.
中科院分区：
文献类型：
--
作者：
Ruzza, A.;Vespignani, R.;Trento, A.
目标。描述了在大鼠异位心脏移植模型中最新的麻醉和手术技术。通过肾上入路实施了显微外科技术，并以逐步的方式进行了描述，并将几项技术改进与先前的描述进行了比较。以Lewis大鼠为供体和受体(同基因模型)。影响早期手术结果的因素包括使用的麻醉剂的类型；外科医生的技能、处理血管的经验和小动物解剖学知识；术中的温和操作；以及所有9只存活的动物都获得了手术持续时间(11个月)的功能正常的移植物。给予异氟醚麻醉，外科医生的微血管手术技能和小动物解剖知识，以及手术持续时间少于1小时，大大提高了患者的存活率。这些因素共同促成了大鼠异位心脏移植后早期的成功结果，90%的患者免于发病和死亡，并在同基因模型中导致了功能正常的移植物的长期存活(>；11个月)。这种大鼠异位模型适用于心脏移植的短期和长期研究。
Objective. An updated anesthetic and surgical technique in a rat model of heterotopic heart transplantation is described.Materials and Methods. A microsurgical technique via a suprarenal approach was performed, and is described in stepwise fashion, and several technical improvements are compared with previous descriptions. Lewis rats were used as donors and recipients (syngeneic model).Results. Factors that affected early surgical outcome included type of anesthetic used; surgeon skill, experience in handling blood vessels, and knowledge of small-animal anatomy; gentle manipulation during the operation; and duration of surgery (11 months) with a functioning graft was achieved in all 9 surviving animals.Conclusions. Survival was substantially improved with administration of isoflurane anesthesia; surgeon microvascular surgical skills and knowledge of small-animal anatomy, and duration of surgery less than 1 hour. These factors collectively contributed to successful early outcomes after heterotopic heart transplantation in rats, with 90% freedom from morbidity and mortality, and resulted in long-term survival (>11 months) with a functioning graft in a syngeneic model. This heterotopic model in rats is suitable for short- and long-term studies of heart transplantation.