Inventing a sex-specific gene:: A conserved role of DMRT1 in teleost fishes plus a recent duplication in the medaka Oryzias latipes resulted in DMY
Inventing a sex-specific gene:: A conserved role of DMRT1 in teleost fishes plus a recent duplication in the medaka Oryzias latipes resulted in DMY
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DOI：
10.1007/s00239-003-0021-4
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发表时间：
2003-01-01
影响因子：
3.9
通讯作者：
Robinson-Rechavi, M
中科院分区：
文献类型：
--
作者：
Lutfalla, G;Roest Crollius, H;Robinson-Rechavi, M
DMY是第一个在非哺乳动物脊椎动物中被描述的性别决定基因，最近在medaka鱼（Oryzias latipes）中被发现。它与DMRT1同源，DMRT1是脊椎动物性别决定级联的保守基因。我们已经检查了另外两种表现异型鱼类（黑病毒四齿鱼和红鳍东鲀）的接近完整的基因组，以寻找DMRT I和DMY的补充同源物。我们还将新基因DMY与其来自所有可用脊椎动物的同源基因DMRT1进行了比较。最后，我们发现了黑病毒绦虫同源物的性别特异性表达和选择性剪接的证据。我们的研究结果表明，DMY是medaka中DMRT1的最近重复。它在性别决定中的作用不是通过进化速度的加速而获得的，而是通过易位到Y染色体以及可能在关键位置的改变而获得的。
DMY, the first sex-determining gene to be described in a nonmammal vertebrate was recently characterized in the medaka fish (Oryzias latipes). It is homologous to DMRT1, a conserved gene of the sex determination cascade in vertebrates. We have checked the near complete genomes of two other percomorph fishes, Tetraodon nigroviridis and Takifugu rubripes, for supplementary homologs of DMRT I and DMY. We also compared the new gene, DMY, to its homolog DMRT1 from all available vertebrates. Finally, we found evidence for sex-specific expression and alternative splicing of the homolog from T. nigroviridis. Our results show that DMY is a recent duplicate of DMRT1 in the medaka. Its role in sex determination was not acquired through an acceleration of evolutionary rates, but by translocation to the Y chromosome and possibly changes at key positions.