Direct observation of tiers in the energy landscape of a chromoprotein:: A single-molecule study
Direct observation of tiers in the energy landscape of a chromoprotein:: A single-molecule study
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DOI：
10.1073/pnas.2533896100
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发表时间：
2003-12-23
影响因子：
11.1
通讯作者：
Köhler, J
中科院分区：
文献类型：
--
作者：
Hofmann, C;Aartsma, TJ;Köhler, J
单分子光谱技术适用于嵌入色素蛋白中的单个色素。通过记录颜料吸收的光谱位置随时间的变化，提供了一种监测发色团局部环境中蛋白质主链结构波动的敏感工具。这些数据提供了有关蛋白质能量景观组织的信息，这些能量景观可以通过平均势垒高度来表征。此外，还观察到不同层内的平均势垒高度与结构波动的时间尺度之间的相关性。
Single-molecule spectroscopic techniques were applied to individual pigments embedded in a chromoprotein. A sensitive tool to monitor structural fluctuations of the protein backbone in the local environment of the chromophore is provided by recording the changes of the spectral positions of the pigment absorptions as a function of time. The data provide information about the organization of the energy landscape of the protein in tiers that can be characterized by an average barrier height. Additionally, a correlation between the average barrier height within a distinct tier and the time scale of the structural fluctuations is observed.