Cancer stem cell marker expression and methylation status in patients with colorectal cancer.
Cancer stem cell marker expression and methylation status in patients with colorectal cancer.
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结直肠癌患者肿瘤干细胞标志物的表达和甲基化状态
DOI：
10.3892/ol.2022.13352
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发表时间：
2022-07
期刊：
影响因子：
2.9
通讯作者：
中科院分区：
文献类型：
--
作者：
在过去十年中，被诊断患有结直肠癌（CRC）的人数呈惊人的上升趋势。在一些国家，这种癌症是最常诊断的肿瘤类型之一。因此，研究该疾病的病理基础，以深入了解耐药机制是一个重要的需求。肿瘤对化疗的抵抗和肿瘤的侵袭性与一小部分肿瘤细胞有关，这被认为是肿瘤复发的原因。这些类型的肿瘤细胞被称为癌症干细胞，它们在这种恶性疾病的发病机制中起着重要作用。斯洛伐克是全世界CRC发病率最高的国家之一。在本研究中，目的是利用流式细胞术对CRC肿瘤中选定的干细胞标记物（CD133、CD166和Lgr5）的丰度进行分类。此外，在斯洛伐克马丁大学医院的一组患者中，通过焦磷酸测序研究了CRC生物标志物（ADAMTS16、MGMT、PROM1 （CD133）、LGR5和ALCAM）选定基因组区域的甲基化状态。对原发肿瘤和转移肿瘤的样本进行了检测。研究人员还分析了5种CRC生物标志物基因组区域的DNA甲基化，发现A分解素和金属蛋白酶（含血栓反应蛋白基元16）和o6 -甲基鸟嘌呤-DNA甲基转移酶基因的甲基化水平最高，而表达突出蛋白-1、富含亮氨酸的重复- g蛋白偶联受体5和活化的白细胞细胞粘附分子的基因的甲基化水平最低。此外，与原发肿瘤组织相比，转移瘤组织中CD133+细胞水平显著升高。较高水平的CD133+细胞与TNM分期和结直肠癌对淋巴系统的侵袭有关。虽然处理的样本数量相对较少，但CD133标记物被认为是CRC病理中的重要标记物。
The number of individuals diagnosed with colorectal cancer (CRC) has been on an alarming upward trajectory over the past decade. In some countries, this cancer represents one of the most frequently diagnosed types of neoplasia. Therefore, it is an important demand to study the pathology underlying this disease to gain insights into the mechanism of resistance to treatment. Resistance of tumors to chemotherapy and tumor aggressiveness have been associated with a minor population of neoplastic cells, which are considered to be responsible for tumor recurrence. These types of neoplastic cells are known as cancer stem cells, which have been previously reported to serve an important role in pathogenesis of this malignant disease. Slovakia has one of the highest incidence rates of CRC worldwide. In the present study, the aim was to classify the abundance of selected stem cell markers (CD133, CD166 and Lgr5) in CRC tumors using flow cytometry. In addition, the methylation status of selected genomic regions of CRC biomarkers (ADAMTS16, MGMT, PROM1 (CD133), LGR5 and ALCAM) was investigated by pyrosequencing in a cohort of patients from Martin University Hospital, Martin, Slovakia. Samples from both primary tumors and metastatic tumors were tested. Analysis of DNA methylation in the genomic regions of indicated five CRC biomarkers was also performed, which revealed the highest levels of methylation in the A disintegrin and metalloproteinase with thrombospondin motifs 16 and O6-methyguanine-DNA methyl transferase genes, whereas the lowest levels of methylation were found in genes expressing prominin-1, leucine-rich repeat-containing G-protein-coupled receptor 5 and activated leukocyte cell adhesion molecule. Furthermore, tumor tissues from metastases showed significantly higher levels of CD133+ cells compared with that in primary tumors. Higher levels of CD133+ cells correlated with TNM stage and the invasiveness of CRC into the lymphatic system. Although relatively small number of samples was processed, CD133 marker was consider to be important marker in pathology of CRC.
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影响因子：
8.8
作者：
通讯作者：
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CD166 表达在结直肠癌中的临床病理学、预后和预测价值：一项荟萃分析
影响因子：
--
作者：
Han S;Yang W;Zong S;Li H;Liu S;Li W;Shi Q;Hou F
通讯作者：
Hou F
CD133 表达与结直肠癌的预后：系统回顾和荟萃分析
影响因子：
3.7
作者：
Chen S;Song X;Chen Z;Li X;Li M;Liu H;Li J
通讯作者：
Li J
DOI：
10.1073/pnas.0703478104
发表时间：
2007-06-12
影响因子：
11.1
作者：
Dalerba, Piero;Dylla, Scott J.;Clarke, Michael F.
通讯作者：
Clarke, Michael F.
影响因子：
29.4
作者：
Levin TG;Powell AE;Davies PS;Silk AD;Dismuke AD;Anderson EC;Swain JR;Wong MH
通讯作者：
Wong MH