ELASTIN IN ALCOHOLIC LIVER-DISEASE - AN IMMUNOHISTOCHEMICAL AND IMMUNOELECTRON MICROSCOPIC STUDY
ELASTIN IN ALCOHOLIC LIVER-DISEASE - AN IMMUNOHISTOCHEMICAL AND IMMUNOELECTRON MICROSCOPIC STUDY
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DOI：
10.1016/s0344-0338(11)80232-3
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发表时间：
1990-10-01
影响因子：
2.8
通讯作者：
GRIMAUD, JA
中科院分区：
文献类型：
--
作者：
PORTO, LC;CHEVALLIER, M;GRIMAUD, JA
Increased elastic stained material has been described in fibronic and cirrhotic liver processes. The aim of this work was to follow the development and distribution of elastic fibers from 48 chronic alcoholic patients. Patients were scored for fibrosis as 0, without fibrosis or minimal (n = 5); 1, incipient or early fibrosis (n = 9); 2, fibrosis or incomplete cirrhosis (n = 12); and 3, cirrhosis (n = 22). Elastica staining was performed by orcein, resorcin-fuchsin and iron hematoxylin and confirmed by immunofluorescence staining with an anti-human elastin antibody (Institut Pasteur). Electron microscopy of representative cases of each group and electron microscopy of immunolabelled elastin (n = 5) were also performed. In early alcoholic fibrosis, oxytalan fibers were pointed out in terminal hepatic veins and in Disse space. In fibrous portal extensions and cirrhotic internodular septa, oxytalan and elaunin fibers represented the major elastin components in association with the alcoholic liver fibroplasia. Immunostaining with anti-elastin Ab exhibits the same distribution as with histochemical methods in portal and septal zones. Electron microscopy confirmed abundant microfibrillar bundles between collagen fibers that mesh and are in continuity withe elaunin fibers. Immunoelectron microscopy confirmed elastin deposits in the amporphous material and in association with the microfibrillar material in the portal and septal zones and disclosed elastin even in the thin strands of fibrotic tissue. In conclusion, elastogenesis, mainly represented by oxytalan and elaunin fibers, develops in alcoholic disease and takes part, with collagen deposits, in the fibrotic process.
