Dimethyl-α-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm
Dimethyl-α-cyclodextrin induces capacitation by removing phospholipids from the plasma membrane of mouse sperm
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二甲基-α-环糊精通过去除小鼠精子质膜上的磷脂诱导获能
DOI：
10.1093/biolre/ioad013
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发表时间：
2023
影响因子：
3.6
通讯作者：
Takeo Toru
中科院分区：
文献类型：
--
作者：
Nakao Satohiro;Ito Kotono;Sakoh Kazuhito;Takemoto Kenji;Watanabe Hitomi;Kondoh Gen;Irie Tetsumi;Nakagata Naomi;Takeo Toru
获能是哺乳动物精子完成受精过程中的重要事件。胆固醇流出是获能的触发器。通常，白蛋白和β-环糊精的胆固醇受体用于在体外受精期间诱导获能。在此之前，我们报道了由七种葡萄糖组成的甲基-β-环糊精（MBCD）在冷冻解冻的小鼠精子中具有比牛血清白蛋白（BSA）更高的诱导获能能力。比较白蛋白和环糊精对获能的影响有助于理解获能的机制。在这项研究中，我们研究了白蛋白，MBCD，和不同类型的环糊精，二甲基-α-环糊精（DMACD），这是由六种葡萄糖，对精子获能的几个事件的影响。结果表明，DMACD可诱导精子获能，提高受精能力。提高受精率所需的时间在BSA、MBCD和DMACD之间不同。BSA和MBCD增强胆固醇和磷脂流出，而DMACD增强磷脂流出。BSA、MBCD和DMACD可增加精子膜流动性、脂筏重排和顶体反应。这些发现表明，磷脂流出是一种新的获能触发。增加精子获能诱导剂的选择可能有助于改善体外受精（IVF）技术，不仅在小鼠中，而且在各种难以通过IVF产生胚胎的物种中。
Capacitation is an important event in the completion of fertilization by mammalian sperm. Cholesterol efflux is a trigger of capacitation. In general, cholesterol acceptors of albumin and β-cyclodextrins are used to induce capacitation during in vitro fertilization. Previously, we reported that methyl-β-cyclodextrin (MBCD), which is composed of seven glucoses, had a higher ability to induce capacitation than bovine serum albumin (BSA) in frozen–thawed mouse sperm. Comparison of albumin and cyclodextrins is helpful for understanding the mechanism of capacitation. In this study, we examined the effects of albumin, MBCD, and a different type of cyclodextrin, dimethyl-α-cyclodextrin (DMACD), which is composed of six glucoses, on several events of sperm capacitation. We showed that DMACD induced sperm capacitation and promoted fertilization ability. The time required to increase the fertilization rate differed among BSA, MBCD, and DMACD. BSA and MBCD enhanced cholesterol and phospholipid efflux, whereas DMACD enhanced only phospholipid efflux. BSA, MBCD, and DMACD increased sperm membrane fluidity, rearrangement of the lipid raft, and the acrosome reaction. These findings suggest that phospholipid efflux is a novel trigger of capacitation. Increasing the choice of sperm capacitation inducers may be useful for improving in vitro fertilization (IVF) techniques not only in mice, but also in various species in which it has been difficult to produce embryos by IVF.
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体外和体内获能后大鼠精子附睾糖蛋白的重新分布
影响因子：
2.5
作者：
L. Rochwerger;P. Cuasnicú
通讯作者：
P. Cuasnicú
影响因子：
18.2
作者：
S. Reynolds
通讯作者：
S. Reynolds
影响因子：
--
作者：
Selvaraj, Vimal;Buttke, Danielle E.;Travis, Alexander J.
通讯作者：
Travis, Alexander J.
DOI：
10.1172/jci16392
发表时间：
2002-09
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
A. Travis;G. Kopf
通讯作者：
A. Travis;G. Kopf
影响因子：
1.7
作者：
Hao, Tong;Zhang, Peipei;Zhao, Xueming
通讯作者：
Zhao, Xueming