Developmental transcriptomes of the sea star, Patiria miniata, illuminate how gene expression changes with evolutionary distance
Developmental transcriptomes of the sea star, Patiria miniata, illuminate how gene expression changes with evolutionary distance
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DOI：
10.1038/s41598-019-52577-9
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发表时间：
2019-11-07
影响因子：
4.6
通讯作者：
De-Leon, Smadar Ben-Tabou
中科院分区：
文献类型：
--
作者：
Gildor, Tsvia;Cary, Gregory A.;De-Leon, Smadar Ben-Tabou
了解发育基因表达的变化如何改变形态发生是发育和进化中的一个基本问题。解决这一问题的一个有希望的方法是比较相关物种之间的发育转录本。棘皮动物门由几个模式物种组成，这些模式物种对了解基因调节和进化做出了重大贡献。特别是海星Patiria mini ata(P.mini ata)的调控网络已被广泛研究，但缺乏该物种的发育转录本。在这里，我们产生了小海胆的发育转录本，并将这些转录本与两种海胆的转录本进行了比较。我们证明，基因表达的保守性取决于基因功能、细胞类型和进化距离。随着进化距离的增加，基因表达的种间相关性降低。在形态上等价的阶段(对角元素)之间的相关性中的减少比在形态上不同的阶段之间的相关性(非对角元素)中的减少更严重。这可能反映了与其他影响基因表达的进化差异较大的限制因素相比，形态限制因素的减少。在这一趋势下，发育控制基因的种间相关性在较大的进化距离内保持其对角性，并在原肠胚形成开始时达到峰值，支持系统发育阶段保护的沙漏模型。
Understanding how changes in developmental gene expression alter morphogenesis is a fundamental problem in development and evolution. A promising approach to address this problem is to compare the developmental transcriptomes between related species. The echinoderm phylum consists of several model species that have significantly contributed to the understanding of gene regulation and evolution. Particularly, the regulatory networks of the sea star, Patiria miniata (P. miniata), have been extensively studied, however developmental transcriptomes for this species were lacking. Here we generated developmental transcriptomes of P. miniata and compared these with those of two sea urchins species. We demonstrate that the conservation of gene expression depends on gene function, cell type and evolutionary distance. With increasing evolutionary distance the interspecies correlations in gene expression decreases. The reduction is more severe in the correlations between morphologically equivalent stages (diagonal elements) than in the correlation between morphologically distinct stages (off-diagonal elements). This could reflect a decrease in the morphological constraints compared to other constraints that shape gene expression at large evolutionary divergence. Within this trend, the interspecies correlations of developmental control genes maintain their diagonality at large evolutionary distance, and peak at the onset of gastrulation, supporting the hourglass model of phylotypic stage conservation.