PRODUCTION BY BACILLIS-SUBTILIS OF BROWN SPORULATION-ASSOCIATED PIGMENTS
PRODUCTION BY BACILLIS-SUBTILIS OF BROWN SPORULATION-ASSOCIATED PIGMENTS
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DOI：
10.1139/m83-015
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发表时间：
1983-01-01
影响因子：
2.8
通讯作者：
HAGEMAN, JH
中科院分区：
文献类型：
--
作者：
BARNETT, TA;VALENZUELA, D;HAGEMAN, JH
The mode of production of the brown pigments of B. subtilis 168 L-4, pigments frequently used as phenotypic markers for sporulation in this organism, was studied. A defined liquid medium which promoted maximal pigment formation was developed. Five brown components, which could be resolved by TLC, were produced in the culture broth. Removal of cells from the medium at the end of logarithmic growth did not alter the type or amount of the pigments formed, indicating that the cells excreted pigment precursors into the medium during growth. Pigment formation from the precursors was found to occur by an O2-requiring, base-dependent, Mn2+-requiring, nonenzymatic pathway. Pigment production was also stimulated by the presence of tyrosine and histidine in the medium. The increases in extracellular pH often associated with spore formation in B. subtilis might be the cause of the concomitant appearance of brown pigments.
