A STUDY OF ORGANIC MATERIAL OF HENS-EGG SHELL
A STUDY OF ORGANIC MATERIAL OF HENS-EGG SHELL
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DOI：
10.1042/bj0820352
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发表时间：
1962-01-01
影响因子：
4.1
通讯作者：
BALCH, DA
中科院分区：
文献类型：
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作者：
BAKER, JR;BALCH, DA
采用乙二胺四乙酸脱钙法制备了鸡蛋壳基质、角质层和膜。基质主要由蛋白质-多糖复合物组成，所述蛋白质-多糖复合物含有11%的多糖和至少70%的蛋白质。基质蛋白的特征在于不存在羟脯氨酸、低浓度的芳香族和含硫氨基酸以及存在约两倍于碱性氨基酸的二羧酸氨基酸。硫酸软骨素A和B存在于基质中，占总多糖的35%。一些半乳糖胺以不同于硫酸软骨素的形式与葡糖胺、半乳糖、甘露糖和岩藻糖一起沿着存在。半乳糖的浓度大于甘露糖的浓度，并且存在微量岩藻糖。氨基己糖与中性糖的摩尔比接近1：1，但没有关于它们结合形式的信息。膜主要是角蛋白和少量的多糖，含有氨基己糖、半乳糖和可能的甘露糖。部分或全部多糖可能来源于乳头核。角质层的大约90%是蛋白质，其通过其酪氨酸、甘氨酸、赖氨酸和胱氨酸的含量而与膜和基质蛋白质区分开。己糖胺与半乳糖、甘露糖和岩藻糖一起存在。
Hen''s-egg-shell matrix, cuticle and membranes were prepared separately by decalcification of the shell with ethylenediaminetetraacetic acid. The matrix consists mainly of a protein -polysaccharide complex containing 11% of polysaccharide and at least 70% of protein. The matrix protein is characterized by the absence of hydroxyproline, the low concentration of aromatic and sulphur-containing amino acids and the presence of about twice as much dicarboxylic amino acids as basic amino acids. Chondroitin sulphates A and B are present in the matrix and comprise 35% of the total polysaccharide. Some galactosamine is present in a form other than chondroitin sulphate along with glucosamine, galactose, mannose and fucose. The concentration of galactose is greater than that of mannose, and fucose is present in trace amounts. The molar ratio hexosamine:neutral sugar is close to 1:1, but no information is available as to the form(s) in which they are combined. The membranes are mainly keratin with a small amount of polysaccharide containing hexosamine, galactose and probably mannose. Part of all of the polysaccharide may originate from the mammillary cores. Some 90% of the cuticle is protein, which is distinguished from the membrane and matrix proteins by its content of tyrosine, glycine lysine and cystine. Hexosamine is present together with galactose, mannose and fucose.