The alteration of SHARPIN expression in the mouse brainstem during herpes simplex virus 1-induced facial palsy
The alteration of SHARPIN expression in the mouse brainstem during herpes simplex virus 1-induced facial palsy
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DOI：
10.1016/j.neulet.2014.12.008
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发表时间：
2015-01
影响因子：
2.5
通讯作者：
Yue Li;Jian-feng Li;Y. Mao;Xiaofei Li;Wenwen Liu;Lei Xu;Yue-chen Han;Haibo Wang
中科院分区：
文献类型：
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作者：
Yue Li;Jian-feng Li;Y. Mao;Xiaofei Li;Wenwen Liu;Lei Xu;Yue-chen Han;Haibo Wang
贝尔氏麻痹表现为面部单侧无力或瘫痪，这是由于周围面神经的急性功能障碍造成的，原因不明。虽然数据显示1型单纯疱疹病毒（HSV-1）可能是贝尔麻痹的病因，但麻痹的确切机制仍不清楚。shank相关的RH结构域相互作用蛋白（SHARPIN）被认为在炎症反应的控制中发挥作用。为了阐明SHARPIN参与小鼠HSV-1所致面瘫的分子通路及皮质类固醇的抑制作用，我们采用4周龄接种HSV-1的Balb/c小鼠进行实验。采用实时定量聚合酶链反应、western blot和免疫荧光法分别检测SHARPIN在脑干面神经核中的表达和定位。在面瘫表现后，在脑干面神经核SHARPIN的表达水平升高，在第2天达到峰值，随后下降。给药后，峰值SHARPIN蛋白表达下调。我们的结果表明，SHRPIN在hsv -1诱导的面瘫的炎症反应中被激活。MPSS可以有效抑制SHARPIN的表达，SHARPIN可能有助于减轻hsv -1介导的神经系统损伤。
Bell’s palsy presents a unilateral weakness or paralysis of the face due to acute dysfunction of the peripheral facial nerve with no readily identifiable cause. Although data show that herpes simplex virus type 1 (HSV-1) may be the possible causative agent of Bell’s palsy, the precise mechanism of the paralysis is still unknown. SHANK-associated RH domain-interacting protein (SHARPIN) is thought to play a role in the control of inflammatory responses. In order to clarify the molecular pathway of SHARPIN involved in the facial palsy caused by HSV-1 in mice and the inhibitory effect of corticosteroids, we used 4-week-old Balb/c mice inoculated with HSV-1 for experiments. The expression and location of SHARPIN in the facial nucleus of brainstem were detected respectively by quantitative real-time polymerase chain reaction, western blot and immunofluorescence. Expression level of SHARPIN increased and peaked at 2 days and then decreased in the facial nucleus of brainstem after the manifestation of the facial paralysis. After the administration of MPSS, the protein expression of SHARPIN at the peak point was down-regulated. Our results suggest that SHRPIN were activated during the inflammatory reaction in the HSV-1-induced facial paralysis. MPSS can effectively inhibit the expression of SHARPIN that may contribute to attenuate HSV-1-mediated nervous system damage.