Effect of Operational Parameters in the Continuous Anaerobic. Fermentation of Cheese Whey on Titers, Yields, Productivities, and Microbial Community Structures
Effect of Operational Parameters in the Continuous Anaerobic. Fermentation of Cheese Whey on Titers, Yields, Productivities, and Microbial Community Structures
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DOI：
10.1021/acssuschemeng.6b01901
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发表时间：
2017-02-01
影响因子：
8.4
通讯作者：
Bertin, Lorenzo
中科院分区：
文献类型：
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作者：
Domingos, Joana M. B.;Martinez, Gonzalo A.;Bertin, Lorenzo
以干酪乳清为原料制备挥发性脂肪酸。建立了一个混合培养填充床生物反应器，在产酸条件下厌氧消化奶酪乳清粉水溶液。批量试验指出，整个VFAs生产过程经过两个连续阶段：(a)将乳糖转化为乳酸和(b)将乳酸转化为VFAs的混合物。此外，同样的试验表明，陶瓷材料Vukopor S10可以作为厌氧发酵过程中细胞固定化的有效载体。在连续运行的台式生物反应器中，研究了水力停留时间（HRT）和有机负载率（OLR）的影响。HRT为6 d， ORL为4.2 g/L/d， pH为5.8-6,16 g。产总VFAs的L-1，产率高于75% （Cmol（VFAs）中心点Cmol（乳糖）（-1））。基于illumina测序的表征表明，当生物膜反应器中发育的微生物群落结构在少数属中高度富集时，获得了较高的VFAs生产率。
Volatile fatty acids (VFAs) were produced using cheese whey as feedstock. A mixed culture packed bed bioreactor was set up to digest anaerobically, under an acidogenic condition, a water solution of a cheese whey powder. Batch tests pointed out that the whole VFAs production process occurred via two sequential phases: (a) conversion of lactose into lactic acid and (b) conversion of lactic acid into a mixture of VFAs. Furthermore, the same tests showed that the ceramic material Vukopor S10 can be used as an effective support for cell immobilization in anaerobic fermentation processes. The effect of the hydraulic retention time (HRT) and organic loading rate (OLR) were then studied in a benchtop bioreactor operated continuously. By a HRT of 6 days, ORL of 4.2 g/L/d, and pH 5.8-6, 16 g.L-1 of total VFAs were produced, with a yield higher than 75% (Cmol(VFAs)center dot Cmol(lactose)(-1)). Characterization with Illumina-based sequencing suggested that high VFAs productivities were obtained when microbial community structures developed in the biofilm reactor were highly enriched in few genera.