Characterization of the in vitro macrophage response and in vivo host response to poly(ethylene glycol)-based hydrogels
Characterization of the in vitro macrophage response and in vivo host response to poly(ethylene glycol)-based hydrogels
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DOI：
10.1002/jbm.a.32595
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发表时间：
2010-06-01
影响因子：
4.9
通讯作者：
Bryant, Stephanie J.
中科院分区：
文献类型：
--
作者：
Lynn, Aaron D.;Kyriakides, Themis R.;Bryant, Stephanie J.
光聚合聚乙二醇（PEG）基水凝胶作为组织工程体内细胞递送载体具有巨大潜力。然而，他们在 vim 中的成功将取决于主机的响应。本研究的目的是探讨常用的基于 PEG 的水凝胶、PEG 和含有 RGD 的 PEG 的体内宿主反应和体外巨噬细胞反应。将无细胞水凝胶皮下植入 c57bl/6 小鼠体内，并将异物反应 (FBR) 与医用级硅胶进行比较。我们的研究结果表明，PEG-RGD 水凝胶产生与硅树脂类似的 FOR，而纯 PEG 水凝胶会产生强烈的炎症反应，其特征是材料表面形成厚厚的巨噬细胞层，并有凝胶降解的证据。在体外，培养 4 天时，骨髓源性原代巨噬细胞粘附良好，与基于 PEG 的水凝胶、硅胶和组织培养聚苯乙烯类似。在 1 天和 2 天时，与 PEG-RGD 和硅胶相比，接种到 PEG 上的巨噬细胞中发现促炎细胞因子 TNF-α 和 Il-1 beta 的基因表达显着升高。 PEG 水凝胶还被证明易于氧化生物降解。我们的研究结果表明，纯 PEG 水凝胶具有促炎性，而 RGD 减弱了这种负面反应，导致中等的 FBR。 (C) 2009 Wiley periodicals, Inc. J Biomed Mater Res 93A: 941-953, 2010
Photopolymerizable poly(ethylene glycol) (PEG)based hydrogels have great potential as to vivo cell delivery vehicles for tissue engineering. However, their success in vim will be dependent on the host response. The objectives for this study were to explore the in vivo host response and in vitro macrophage response to commonly used PEG-based hydrogels, PEG and PEG containing RGD. Acellular hydrogels were implanted subcutaneously into c57bl/6 mice and the foreign body response (FBR) was compared to medical grade silicone. Our findings demonstrated PEG-RGD hydrogels resulted in a FOR similar to silicone, while PEG-only hydrogels resulted in a robust inflammatory reaction characterized by a thick layer of macrophages at the material surface with evidence of gel degradation. In vitro, bone marrow-derived primary macrophages adhered well and similarly to PEG-based hydrogels, silicone, and tissue culture polystyrene when cultured for 4 days. Significantly higher gene expressions of the proinflammatory cytokines, TNF-alpha and Il-1 beta, were found in macrophages seeded onto PEG compared to PEG-RGD and silicone at 1 and 2 days. PEG hydrogels were also shown to be susceptible to oxidative biodegradation. Our findings indicate that PEG-only hydrogels are proinflammatory while RGD attenuates this negative reaction leading to a moderate FBR. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 93A: 941-953, 2010