Human platelets express gangliosides with LKE activity and ABH blood group activity
Human platelets express gangliosides with LKE activity and ABH blood group activity
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DOI：
10.1046/j.1537-2995.2001.41040504.x
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发表时间：
2001-04-01
期刊：
影响因子：
2.9
通讯作者：
Koerner, TAW
中科院分区：
文献类型：
--
作者：
Colling, LLW;Zhang, DS;Koerner, TAW
背景技术背景：血小板表达几种具有ABH和P血型活性的中性鞘糖脂，可能在感染性、自身免疫性和同种免疫性血小板减少症中发挥作用。在RBC中，也报告了具有血型活性的唾液酸化鞘糖脂或神经节苷脂。要确定是否类似的抗原表达的血小板，总血小板神经节苷脂馏分分离和筛选血型活性glycosphingolipids.Study设计和方法：血小板神经节苷脂分离有机提取，碱水解，阴离子交换，反式酸，和高效液相色谱法。结果：A组血小板表达5种具有A组活性的神经节苷脂，而O组血小板不表达。在检测的5种A MoAbs和凝集素中，只有MoAbs Birma-1和MHO 4识别所有5种唾液酸A条带。唾液酸A带对神经酰胺内切酶和神经氨酸酶敏感。一条唾液酸A带可能代表具有唾液酸-I和A组活性的分支神经节苷脂。血小板还表达与sialyl-galactosylgloboside.CONCLUSION一致的LKE活性神经节苷脂：除sialyl-il和sialyl-Le（x）神经节苷脂外，A组血小板还表达具有LKE活性和A组活性的神经节苷脂。像红细胞一样，A组活性神经节苷脂可以作为同种异体抗原和自身抗原天然存在的同种血凝素。
BACKGROUND: Platelets express several neutral glycosphingolipids with ABH and P blood group activity that may play a role in infectious, autoimmune, and alloimmune thrombocytopenia. In RBCs, sialylated glycosphingolipids or gangliosides with blood group activity have also been reported. To determine whether similar antigens are expressed by platelets, the total platelet ganglioside fraction was isolated and screened for blood-group-active glycosphingolipids.STUDY DESIGN AND METHODS: Platelet gangliosides were isolated by organic extraction, base hydrolysis, anion exchange, silicic acid, and high-performance liquid chromatography. Gangliosides were identified and characterized by high-performance thin-layer chromatography-immunostaining with blood group-specific MoAbs and glycosidase digestion.RESULTS: Group A, but not group O, platelets express five gangliosides with group A activity. Of five A MoAbs and lectins examined, only MoAbs Birma-1 and MHO4 recognized all five sialyl A bands. The sialyl A bands were sensitive to endoglycoceramidase and neuraminidase. One sialyl A band may represent a branched gan glioside with sialyl-l and group A activity. Platelets also express an LKE-active ganglioside consistent with sialyl-galactosylgloboside.CONCLUSION: In addition to sialyl-il and sialyl-Le(x) gangliosides, group A platelets express gangliosides with LKE activity and group A activity. Like RBCs, group A-active gangliosides may act as alloantigens and autoantigens to naturally occurring isohemagglutinins.