Effect of interferon on the replication of mink cell focus-inducing virus in murine cells: synthesis, processing, assembly, and release of viral proteins
Effect of interferon on the replication of mink cell focus-inducing virus in murine cells: synthesis, processing, assembly, and release of viral proteins
复制标题
干扰素对小鼠细胞中水貂细胞焦点诱导病毒复制的影响：病毒蛋白的合成、加工、组装和释放
作者：
J. Bilello;N. Wivel;P. Pitha
用小鼠成纤维细胞干扰素（150至600 U/ml）处理水貂细胞病灶诱导（MCF）病毒（分离株AK-13）产生的SC-1细胞，导致感染性病毒释放减少100倍，而病毒颗粒释放的各种参数减少2.5至10倍。标记病毒体蛋白的分析表明，干扰素治疗后病毒体蛋白组成发生了时间变化。在慢性感染细胞暴露于干扰素24小时后，产生的病毒体含有85，000-道尔顿糖蛋白（显然是非病毒来源的），其超过病毒包膜糖蛋白gp 70。用干扰素处理32至48小时的细胞产生的颗粒几乎没有gp 70，并含有大量的p30。在干扰素存在下，病毒前体多聚蛋白到成熟病毒体结构蛋白的细胞内加工没有改变。然而，在干扰素处理的细胞中观察到病毒p30和p12 E蛋白的积累，与细胞相关病毒体的增加一致。从[35 S]蛋氨酸标记的对照和干扰素处理的细胞的组织培养液的免疫沉淀分析显示，干扰素处理后释放的p30和p15 E/p12 E显着减少。相反，gp 70在干扰素处理的细胞中不积累，而是以既不可沉淀也不与p15 E/p12 E相关的形式释放到培养基中。
Treatment of mink cell focus-inducing (MCF) virus (isolate AK-13) producing SC-1 cells with mouse fibroblast interferon (150 to 600 U/ml) led to a 100-fold decrease in the release of infectious virus, whereas there was a 2.5- to 10-fold decrease in various parameters of virus particle release. Analysis of labeled virion proteins indicated that a temporal change in virion protein composition occurred after interferon treatment. After a 24-h exposure of chronically infected cells to interferon, the virions produced contained a 85,000-dalton glycoprotein (apparently of nonviral origin) which was in excess of the virus envelope glycoprotein gp70. Particles produced from cells treated with interferon for 32 to 48 h were nearly devoid of gp70 and contained substantially lower quantities of p30. Intracellular processing of viral precursor polyproteins to the mature virion structural proteins was not altered in the presence of interferon. However, an accumulation of the viral p30 and p12E proteins was observed in interferon-treated cells, consistent with an increase in cell-associated virions. Immunoprecipitation analysis of the tissue culture fluids from [35S]methionine-labeled control and interferon-treated cells revealed marked decrease in p30 and p15E/p12E released after interferon treatment. In contrast, gp70 did not accumulate in interferon-treated cells, but was released into the culture medium in a form that was neither pelletable nor associated with p15E/p12E.
DOI：
10.1089/jir.1981.1.613
发表时间：
1981
期刊：
Journal of interferon research
影响因子：
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作者：
L. Pfeffer;F. Landsberger;I. Tamm
通讯作者：
L. Pfeffer;F. Landsberger;I. Tamm