Purification of hemopoietin 1: a multilineage hemopoietic growth factor.
Purification of hemopoietin 1: a multilineage hemopoietic growth factor.
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纯化造血素 1：一种多谱系造血生长因子。
DOI：
10.1073/pnas.82.9.2764
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发表时间：
1985
影响因子：
11.1
通讯作者：
Stanley,ER
中科院分区：
文献类型：
--
作者：
Jubinsky,PT;Stanley,ER
造血素1 （H-1）和单核吞噬细胞特异性生长因子CSF-1协同作用于发育早期骨髓细胞，生成原始CSF-1受体承载细胞。以人膀胱癌细胞株5637为条件的无血清培养基的H-1活性是由两种大小相近的带电种（pI约等于4.8，约等于85%；pI约等于5.3，约等于15%）的活性之和得出的。两种植物的生物活性没有质的差异。一个四步骤的程序，包括批deae -纤维素层析，层析聚焦，凝胶过滤和疏水层析，已开发的主要物种（pI约等于4.8）。H-1被纯化了大约65000倍，回收率为起始材料总活性的32%。产生最大生物活性的最低浓度约为0.25 ng/ml。125i标记的纯化H-1，无论是天然形式还是还原形式，在十二烷基硫酸钠凝胶电泳（NaDodSO4/PAGE）上表现为均匀的单条带，与纯化H-1的生物活性共电泳。NaDodSO4/PAGE测定的纯化还原H-1的分子量约为17 kDa。最近的研究表明纯化后的H-1是一种多系造血生长因子。
Hemopoietin 1 (H-1) and the mononuclear phagocyte specific growth factor CSF-1 act synergistically on developmentally early bone marrow cells to generate primitive CSF-1 receptor-bearing cells. The H-1 activity of the serum-free medium conditioned by the human urinary bladder carcinoma cell line 5637 was shown to result from the sum of the activities of two charged species (pI approximately equal to 4.8, approximately equal to 85%; pI approximately equal to 5.3, approximately equal to 15%) of similar size. No qualitative difference in the biological activity of these two species was detected. A four-step procedure, involving batch DEAE-cellulose chromatography, chromatofocusing, gel filtration, and hydrophobic chromatography has been developed for the major (pI approximately equal to 4.8) species. H-1 was purified approximately 65,000-fold and recovered as 32% of the total activity of the starting material. The lowest concentration yielding maximal biological activity was approximately equal to 0.25 ng/ml. The 125I-labeled purified H-1, in either native or reduced form, behaved as a homogeneous single band that coelectrophoresed with the biological activity of purified H-1 on sodium dodecyl sulfate gel electrophoresis (NaDodSO4/PAGE). The molecular mass of the purified reduced H-1, determined by NaDodSO4/PAGE was approximately equal to 17 kDa. Recent studies indicate that the purified H-1 is a multilineage hemopoietic growth factor.