A novel virus in swine is closely related to the human hepatitis E virus
A novel virus in swine is closely related to the human hepatitis E virus
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DOI：
10.1073/pnas.94.18.9860
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发表时间：
1997-09-02
影响因子：
11.1
通讯作者：
Emerson, SU
中科院分区：
文献类型：
--
作者：
Meng, XJ;Purcell, RH;Emerson, SU
在猪体内发现了一种新型病毒，称为猪戊型肝炎病毒（猪 HEV），猪 HEV 与人类 HEV 衣壳抗原的抗体发生交叉反应，猪 HEV 是一种普遍存在的病原体，美国中西部猪群中大多数大于或等于 3 月龄的猪呈血清阳性。自然感染猪HEV的幼猪临床表现正常，但有肝炎的微观证据，并在血清转化前出现病毒血症，通过逆转录PCR从自然感染猪的血清中扩增整个ORF 2和3，猪HEV的推定衣壳基因（ORF2）在核苷酸水平上具有约79-80％的序列同一性和90-92％的同一性 在氨基酸水平上与人HEV毒株的小ORF3与人HEV毒株有83-85%的核苷酸序列一致性和77-82%的氨基酸一致性，系统发育分析表明猪HEV与人HEV毒株密切相关，但又不同于人HEV毒株，猪HEV的发现不仅对HEV疫苗的开发、诊断和生物学具有重要意义，而且还提出了 猪器官异种移植后人畜共患病或异种动物病的潜在公共卫生问题。
A novel virus, designated swine hepatitis E virus (swine HEV), was identified in pigs, Swine HEV crossreacts with antibody to the human HEV capsid antigen, Swine HEV is a ubiquitous agent and the majority of swine greater than or equal to 3 months of age in herds from the midwestern United States were seropositive. Young pigs naturally infected by swine HEV were clinically normal but had microscopic evidence of hepatitis, and developed viremia prior to seroconversion, The entire ORFs 2 and 3 were amplified by reverse transcription-PCR from sera of naturally infected pigs, The putative capsid gene (ORF2) of swine HEV shared about 79-80% sequence identity at the nucleotide level and 90-92% identity at the amino acid level with human HEV strains, The small ORF3 of swine HEV had 83-85% nucleotide sequence identity and 77-82% amino acid identity with human HEV strains, Phylogenetic analyses showed that swine HEV is closely related to, but distinct from, human HEV strains, The discovery of swine HEV not only has implications for HEV vaccine development, diagnosis, and biology, but also raises a potential public health concern for zoonosis or xenozoonosis following xenotransplantation with pig organs.