Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program
Development and implementation of a database system to manage a large-scale mouse ENU-mutagenesis program
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DOI：
10.1007/s00335-004-2265-8
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发表时间：
2004-05-01
期刊：
影响因子：
2.5
通讯作者：
Shiroishi, T
中科院分区：
文献类型：
--
作者：
Masuya, H;Nakai, Y;Shiroishi, T
RIKEN GSC 的小鼠 ENU 诱变计划已启动，以对突变小鼠进行大规模、全基因组、早发型和晚发型表型筛选。我们每周对大约一百只小鼠进行一套全面的表型测定，包括基于修改的 SHIRPA 方案的行为测试、血液测试（临床生化测试和血象）以及在家笼中的运动活动测量。为了管理整个程序，我们开发了一个客户端/服务器架构的数据库系统，并将其命名为MUSDB（(M) under bar utagenesis (U) under bar niversal (S) under bar upport (D) under bar ata (B) under bar ase）。它管理小鼠饲养、交配方案、ENU 注射程序和表型筛选、表型遗传测试、精子和器官的保存以及项目期间产生的其他材料。我们已经在一个相当大的系统中实现了 MUSDB，其中包括 150 台客户端计算机。它有助于减少印刷错误，并通过其前端用户界面提供简单高效的操作。它极大地促进了该计划中工作组内部和之间的沟通以及各种表型和遗传数据的积累。
A mouse ENU-mutagenesis program at RIKEN GSC has been initiated to conduct a large-scale, genome-wide, early- and late-onset phenotypic screen of mutant mice. We screened about a hundred mice every week with a comprehensive set of phenotype assays including behavioral tests based on a modified SHIRPA protocol, blood tests (both clinical biochemical testing and hemogram), and measurement of locomotor activity in their home cages. To manage the entire program, we developed a client/server architecture database system and named it MUSDB ((M) under bar utagenesis (U) under bar niversal (S) under bar upport (D) under bar ata (B) under bar ase). It manages mouse husbandry, mating protocols, procedures for ENU injection and phenotypic screens, phenotype inheritance tests, preservation of sperm and organs, and other materials generated during the program. We have implemented MUSDB in quite a large-scale system that includes 150 client computers. it has, helped reduce typographical errors and provided simple and efficient operation via its front-end user interface. It significantly contributed to the communication within and between workgroups in the program and in the accumulation of various phenotypic and inheritance data.