Bacteriostatic activity and cytotoxicity of bacterial cellulose-chitosan film loaded with in-situ synthesized silver nanoparticles
Bacteriostatic activity and cytotoxicity of bacterial cellulose-chitosan film loaded with in-situ synthesized silver nanoparticles
复制标题
原位合成银纳米粒子负载细菌纤维素-壳聚糖膜的抑菌活性和细胞毒性
DOI：
10.1016/j.carbpol.2021.119017
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发表时间：
2022
影响因子：
11.2
通讯作者：
Juanqin Xue
中科院分区：
文献类型：
--
作者：
Hang Zhao;Liang Zhang;Sen Zheng;Shouning Chai;Jinliu Wei;Lvling Zhong;Yingying He;Juanqin Xue
开放性伤口的细菌感染一直威胁着人类的健康。在此，我们采用生物自生成和原位还原的方法制备了银(Ag)-多巴胺(PDA)/细菌纤维素(BC)-壳聚糖(CTS)薄膜。在培养基中加入CTS，使BC在成膜过程中与CTS缠绕在一起。在紫外光照射下，硝酸银被原位还原为银纳米粒子，并在PDA的帮助下很好地分散在BC-CTS膜中，与CTS具有协同作用。Ag-PDA/BC-CTS膜富含官能团，具有良好的拉伸和溶胀性能。该抑菌膜对革兰氏阳性菌和革兰氏阴性菌均表现出广谱抑制作用，连续使用48h后抑制率仍保持在80%以上。用NIH3T3成纤维细胞验证了该膜良好的生物相容性。结果表明，Ag-PDA/BC-CTS膜对有害细菌的生长有抑制作用，而对健康细胞的影响不大。
Bacterial infections on open wounds have always been a threat to human health. Herein, we prepared a silver (Ag)-polydopamine (PDA)/bacterial cellulose (BC)-chitosan (CTS) film using biological self-generation and in situ reduction. CTS was added to culture medium to allow BC to intertwine with CTS during film formation. Silver nitrate was reduced in situ to Ag nanoparticles under ultraviolet irradiation, and the nanoparticles were well dispersed in the BC-CTS film with the help of PDA, which worked synergistically with the CTS. The Ag-PDA/BC-CTS film was enriched in functional groups, and it had good tensile and swelling properties. The inhibition film demonstrated broad-spectrum inhibition of Gram-positive and Gram-negative bacteria, and this inhibition was maintained at more than 80% after 48 h of continuous use. The good biocompatibility of the film was verified using NIH3T3 fibroblasts. The results suggested that the Ag-PDA/BC-CTS film inhibited the growth of harmful bacteria while having little effect on healthy cells.