Genome-Wide Identification and Characterization of ABC Transporters in Nine Rosaceae Species Identifying MdABCG28 as a Possible Cytokinin Transporter linked to Dwarfing
Genome-Wide Identification and Characterization of ABC Transporters in Nine Rosaceae Species Identifying MdABCG28 as a Possible Cytokinin Transporter linked to Dwarfing
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九种蔷薇科物种 ABC 转运蛋白的全基因组鉴定和表征 将 MdABCG28 鉴定为与矮化相关的可能细胞分裂素转运蛋白
DOI：
10.3390/ijms20225783
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发表时间：
2019-11-01
影响因子：
5.6
通讯作者：
Wang, Yi
中科院分区：
文献类型：
--
作者：
Feng, Yi;Sun, Qiran;Wang, Yi
ATP 结合盒 (ABC) 转运蛋白构成了一个庞大、多样且普遍存在的超家族，参与广泛的过程。基因组测序的完成为了解蔷薇科物种中ABC转运蛋白超家族的系统发育历史提供了机会。本研究从9个蔷薇科基因组中鉴定出总共1323个ABC转运蛋白基因：191个来自Malus Domestica，174个来自Pyrus communis，138个来自Prunus persica，118个来自Prunus avium，141个来自Prunus dulcis，122个来自Fragaria vesca，98个来自Rubus occidentalis，162个来自Prunus mume，以及162个来自Prunus mume。 179 来自 月季。研究了它们的化学特征、系统发育分析、染色体定位、基因结构、基因复制和组织特异性表达。预测了它们的亚细胞定位、跨膜结构和蛋白质基序。所有 ABC 转运蛋白基因根据其系统发育关系和结构特征分为八个亚家族。此外，还对MdABCG亚家族基因中10种潜在植物激素转运蛋白进行了顺式元件和表达分析。研究发现，MdABCG28 启动子区域中 W 盒的丢失会降低基因表达水平，并与苹果砧木的矮化表型相关。 MdABCG28 过表达促进了拟南芥中 atabcg14 突变体的芽生长。
ATP-binding cassette (ABC) transporters constitute a large, diverse, and ubiquitous superfamily that is involved in a broad range of processes. The completion of genome sequencing provides an opportunity to understand the phylogenetic history of the ABC transporter superfamily among Rosaceae species. This study identified a total of 1323 ABC transporter genes from nine Rosaceae genomes: 191 from Malus domestica, 174 from Pyrus communis, 138 from Prunus persica, 118 from Prunus avium, 141 from Prunus dulcis, 122 from Fragaria vesca, 98 from Rubus occidentalis, 162 from Prunus mume, and 179 from Rosa chinensis. Their chemical characterization, phylogenetic analysis, chromosomal localization, gene structure, gene duplication, and tissue-specific expression were studied. Their subcellular localization, transmembrane structures, and protein motifs were predicted. All the ABC transporter genes were grouped into eight subfamilies on the basis of their phylogenetic relationships and structural features. Furthermore, cis-element and expression analysis of 10 potential phytohormone transporters in MdABCG subfamily genes were also performed. Loss of the W-box in the promoter region of MdABCG28 was found to reduce the gene expression level and was linked to the dwarfing phenotype in apple rootstocks. MdABCG28 overexpression promoted shoot growth of atabcg14 mutants in Arabidopsis.