The armadillo protein p0071 controls KIF3 motor transport
The armadillo protein p0071 controls KIF3 motor transport
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DOI：
10.1242/jcs.200170
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发表时间：
2017-10-01
影响因子：
4
通讯作者：
Seufferlein, Thomas
中科院分区：
文献类型：
--
作者：
Becher, Alexander;Eiseler, Tim;Seufferlein, Thomas
在这里，我们报告了一种新的功能的犰狳蛋白p0071（也称为PKP4）在运输过程中介导的KIF3运输复合物。胰腺神经内分泌肿瘤细胞或胰腺癌细胞的嗜铬粒蛋白A和基质金属肽酶9的分泌分别在p0071敲低后显著减少。囊泡追踪表明，p0071耗尽后，囊泡运动的方向持久性受损。这表明在缺乏p0071的细胞中，正端和负端定向微管转运之间的平衡受到干扰。p0071直接与KIF3马达亚基KIF3B相互作用。我们的数据表明，p0071还与驱动蛋白货物衔接蛋白KAP 3（也称为KIFAP 3）相互作用，作为KIF3 B和其KAP 3货物结合实体之间的稳定接头。因此，p0071是定向囊泡运动和分泌不同KIF3转运载体所必需的，从而调节细胞内膜囊泡沿着微管的转运。
We here report a novel function of the armadillo protein p0071 (also known as PKP4) during transport mediated by the KIF3 transport complex. Secretion of chromogranin A and matrix metallopeptidase 9 from pancreatic neuroendocrine tumor cells or pancreatic cancer cells, respectively, was substantially reduced following knockdown of p0071. Vesicle tracking indicated that there was impaired directional persistence of vesicle movement upon p0071 depletion. This suggests a disturbed balance between plus- and minus-end directed microtubule transport in cells lacking p0071. p0071 directly interacts with the KIF3 motor subunit KIF3B. Our data indicate that p0071 also interacts with the kinesin cargo adaptor protein KAP3 (also known as KIFAP3) acting as a stabilizing linker between KIF3B and its KAP3 cargo-binding entity. Thus, p0071 is required for directional vesicle movement and secretion of different KIF3-transported carriers, thereby regulating the transport of intracellular membrane vesicles along microtubules.