QTL-based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L.)
QTL-based analysis of leaf senescence in an indica/japonica hybrid in rice (Oryza sativa L.)
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DOI：
10.1007/s00122-005-1955-2
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发表时间：
2005-05-01
影响因子：
5.4
通讯作者：
Ikehashi, H
中科院分区：
文献类型：
--
作者：
Abdelkhalik, AF;Shishido, R;Ikehashi, H
为了鉴定水稻叶片衰老及其相关性状的数量性状位点，以IR 36为籼型亲本，Nekken-2为粳型亲本，构建了籼/粳/粳和籼/粳/籼两个回交群体。利用一组简单重复序列标记（SSR）对BC 1F 1群体中的QTL进行定位。通过测量开花后25天（25天）的叶片叶绿素含量、叶绿素含量的减少（开花时和25天时的叶绿素含量之间的差异）以及来自每个BC 1F 2系的5个植物中25天时每穗的晚变色叶的数量来表征这些植物中的衰老。这些植物在开花时被移入温度受控的生长箱中，并允许在相同的条件下成熟。在两个群体中共检测到11个QTL。衰老QTL主要位于第6染色体短臂和第9染色体长臂上。其中，通过确认基因型对BC 1F 3品系表型的影响，验证了第6染色体上的一个QTL和第9染色体上的两个QTL。粳稻亲本被发现有助于晚衰老，但在所有一个QTL。通过比较杂合子和纯合子对各QTL基因型表型值的影响，我们得出结论：籼粳杂种的衰老差异不是由于超显性，而是双亲中任一方贡献的部分显性基因的结果。
In order to identify quantitative trait loci (QTLs) for leaf senescence and related traits in rice ( Oryza sativa L.), we developed two backcross populations, indica/japonica// japonica and indica/ japonica// indica, using IR36 as the indica parent and Nekken-2 as the japonica parent. The QTLs were mapped using a set of simple sequence-repeat markers (SSRs) in the BC1F1 population. Senescence was characterized in these plants by measuring the leaf chlorophyll content 25 days after flowering (DAF), the reduction in chlorophyll content ( the difference between the chlorophyll content at flowering and at 25 DAF), and the number of late-discoloring leaves per panicle at 25 DAF in five plants from each BC1F2 line. These plants were moved into a temperature-controlled growth cabinet at the time of flowering and allowed to mature under identical conditions. Eleven QTLs were detected in the two populations. The major of QTLs for senescence were found on the short arm of chromosome 6 and on the long arm of chromosome 9. Of these, one QTL on chromosome 6 and two on chromosome 9 were verified by confirming the effects of the genotypes on the phenotypes of the BC1F3 lines. The japonica parent was found to contribute to late senescence at all but one QTL. Based on a comparison of the effects of heterozygotes and homozygotes on the phenotypic values of each QTL genotype, we concluded that the differential senescence observed in the indica-japonica hybrid was not due to over-dominance; rather, it was the result of partial-dominance genes that were donated from either of the parents.