Detection of GII-4/2006b Variant and Recombinant Noroviruses in Children With Acute Gastroenteritis, South Korea
Detection of GII-4/2006b Variant and Recombinant Noroviruses in Children With Acute Gastroenteritis, South Korea
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DOI：
10.1002/jmv.21650
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发表时间：
2010-01-01
影响因子：
12.7
通讯作者：
Hwang, Eung-Soo
中科院分区：
文献类型：
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作者：
Chung, Ju-Young;Han, Tae-Hee;Hwang, Eung-Soo
诺如病毒（Norovirus，NoV）是一种单链、阳性RNA病毒，是世界范围内儿童急性胃肠炎的重要病原体。在这项研究中，从2007年9月至2008年7月在首尔的434名急性胃肠炎儿童中收集了总共434份粪便样本，进行了检测，以确定NoV的分子流行病学，并通过RT-PCR和测序来表征重组菌株。在434份标本中，分别在155份（35.8%）、72份（16.6%）和19份（4.3%）标本中检出NoV、轮状病毒和腺病毒。在7份标本（1.6%）中检测到NoV GI，在148份标本（34.1%）中检测到GII。GII阳性标本中衣壳序列的系统发育分析显示存在以下菌株：GII-4，111（75.0%）; GII-3，35例（23.6%）; GII-6 b，1例;和GII-16，1例。大多数GII-4菌株与具有98-100%核苷酸同一性的GII-4/2006 b变体分组。通过基于RdRP和衣壳基因的测序，11个菌株被鉴定为重组（10例中的GII-4/GII-3和1例中的GII-b聚合酶/GII-16衣壳）。重组菌株中推定的重组点是ORF-1/ORF 2重叠，位于核苷酸5，046处，参考Lordsdale。总之，GII-4/2006 b变异体主要检测到，并发现一个新的重组菌株（GII-4/GII-3）在韩国儿童胃肠炎。持续监测NoV的遗传多样性对于确定优势基因型和新重组菌株的趋势是重要的。J. Med. Virol. 82：146-152，2010. (C)2009 Wiley-Liss，Inc.
Norovirus (NoV), a single-stranded, positive RNA virus, is an important etiologic agent of acute gastroenteritis in children worldwide. In this study, a total of 434 fecal samples collected fro m 434 children with acute gastroenteritis in Seoul, between September 2007 and July 2008 were tested to determine the molecular epidemiology of NoVs and characterize recombinant strains by using RT-PCR followed by sequencing. Of the 434 specimens, NoV, rotavirus, and adenovirus were detected in 155 (35.8%), 72 (16.6%), and 19 specimens (4.3%), respectively. NoV GI was detected in 7 specimens (1.6%) and GII in 148 (34.1%) specimens. Phylogenetic analysis of capsid sequences in the GII-positive specimens revealed the presence of the following strains: GII-4, 111 (75.0%); GII-3, 35 cases (23.6%); GII-6b, 1 case; and GII-16, 1 case. Most of the GII-4 strains were grouped with the GII-4/2006b variant with 98-100% nucleotide identity. Eleven strains were identified as recombinant (GII-4/GII-3 in 10 cases and GII-b polymerase/GII-16 capsid in 1 case) by sequencing based on the RdRP and capsid genes. The putative recombination point in the recombinant strains was the ORF-1/ORF2 overlap, located at nucleotide 5,046 with reference to Lordsdale. In conclusion, GII-4/2006b variants were detected predominantly and a new recombinant strain (GII-4/GII-3) was found in the Korean children with gastroenteritis. Continuous monitoring of the genetic diversity of NoVs is important to determine the trend of the predominant genotype and new recombinant strain. J. Med. Virol. 82:146-152, 2010. (C) 2009 Wiley-Liss, Inc.