Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels.
Using FRET-Based Fluorescent Sensors to Monitor Cytosolic and Membrane-Proximal Extracellular ATP Levels.
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使用基于 FRET 的荧光传感器监测细胞溶质和近膜细胞外 ATP 水平
DOI：
10.1007/978-1-4939-9717-6_16
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发表时间：
2020
影响因子：
--
通讯作者：
Haag F
中科院分区：
文献类型：
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作者：
Kaschubowski KE;Kraft AE;Nikolaev VO;Haag F
在受体结合位点处的细胞外ATP（eATP）的局部浓度的评估仍然是嘌呤能信号传导领域的挑战。在许多情况下，利用福斯特共振能量转移（FRET）原理的生物传感器提供了有用的工具来可视化代谢物的局部浓度。一系列基于细菌ATP酶的FRET的生物传感器已经被描述用于ATP的可视化。这些传感器携带对ATP具有不同亲和力的ATP传感单元，允许在亚细胞位置（例如细胞质和膜近端细胞外空间）中发现的广泛不同浓度条件下对ATP进行成像。
The assessment of local concentrations of extracellular ATP (eATP) at the site of receptor binding remains a challenge in the field of purinergic signaling. In many cases, biosensors exploiting the principle of Förster resonance energy transfer (FRET) have provided useful tools to visualize local concentrations of metabolites. A series of FRET-based biosensors based on the epsilon subunits of bacterial ATP synthases have been described for the visualisation of ATP. These sensors carry ATP-sensing units with different affinities for ATP, permitting imaging of ATP under the widely different concentration conditions found in subcellular locations such as the cytoplasm and the membrane-proximal extracellular space.
DOI：
10.1007/978-1-4939-6810-7_6
发表时间：
2017-01-01
期刊：
LIGHT MICROSCOPY: METHODS AND PROTOCOLS
影响因子：
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作者：
Kraft, Axel E.;Nikolaev, Viacheslav O.
通讯作者：
Nikolaev, Viacheslav O.