Minimally invasive intracellular delivery based on electrokinetic forces combined with vibration-assisted cell membrane perforation
Minimally invasive intracellular delivery based on electrokinetic forces combined with vibration-assisted cell membrane perforation
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DOI：
10.7567/jjap.56.017001
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发表时间：
2017-01-01
影响因子：
1.5
通讯作者：
Nagai, Moeto
中科院分区：
文献类型：
--
作者：
Shibata, Takayuki;Ozawa, Tatsuya;Nagai, Moeto
为了给细胞机制的基础分析和细胞功能的调控提供一个有效的平台，我们开发了一种独特的微创细胞内给药方法。使用这种方法，我们成功地展示了DNA分子通过基于直流偏置交流驱动电动势的玻璃微吸管输送到活的HeLa细胞中，比压力驱动流动方法具有更好的可控性。我们还提出了一种振动辅助插入方法来穿透细胞膜，以减少细胞损伤。初步的插入试验表明，应用机械振荡可以减少细胞由于粘性阻力增加而产生的变形，从而导致细胞膜穿孔和细胞存活的可能性很高。此外，为了克服单一玻璃微吸管细胞内给药的内在低通量，我们开发了一种包括具有明确尖端的阶梯形空心二氧化硅(SiO_2)纳米针阵列的制造工艺。(C)2017日本应用物理学会
To provide an effective platform for the fundamental analysis of cellular mechanisms and the regulation of cellular functions, we developed a unique method of minimally invasive intracellular delivery. Using this method, we successfully demonstrated the delivery of DNA molecules into living HeLa cells via a glass micropipette based on DC-biased AC-driven electrokinetic forces with much better controllability than that of the pressure-driven flow method. We also proposed a vibration-assisted insertion method for penetrating the cell membrane to reduce cell damage. Preliminary insertion tests revealed that application of mechanical oscillation can reduce the deformation of cells due to increases in their viscous resistance, resulting in a high probability of cell membrane perforation and cell viability. Moreover, to overcome the intrinsic low throughput of intracellular delivery with a single glass micropipette, we developed a fabrication process involving an array of stepped hollow silicon dioxide (SiO2) nanoneedles with well-defined tips. (C) 2017 The Japan Society of Applied Physics