BRONCHIAL BIOPSY EVIDENCE FOR LEUKOCYTE INFILTRATION AND UP-REGULATION OF LEUKOCYTE-ENDOTHELIAL CELL-ADHESION MOLECULES 6 HOURS AFTER LOCAL ALLERGEN CHALLENGE OF SENSITIZED ASTHMATIC AIRWAYS
BRONCHIAL BIOPSY EVIDENCE FOR LEUKOCYTE INFILTRATION AND UP-REGULATION OF LEUKOCYTE-ENDOTHELIAL CELL-ADHESION MOLECULES 6 HOURS AFTER LOCAL ALLERGEN CHALLENGE OF SENSITIZED ASTHMATIC AIRWAYS
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DOI：
10.1172/jci117118
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发表时间：
1994-04-01
影响因子：
15.9
通讯作者：
CARROLL, MP
中科院分区：
文献类型：
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作者：
MONTEFORT, S;GRATZIOU, C;CARROLL, MP
我们检查了六名特应性哮喘患者在局部支气管内过敏原激发后 5-6 小时的支气管活检中发生的粘膜变化，并将其与同一时间点来自同一受试者的盐水激发区段的活检进行了比较。所有受试者在过敏原激发的部分均出现局部支气管收缩，并且在激发后 24 小时内 1 秒内用力呼气量 (FEV(1)) (P < 0.01) 减少，并且将 FEV(1) 从基线降低 20% 所需的激动剂乙酰甲胆碱激发浓度降低 (P < 0.05)。 6小时时，我们观察到中性粒细胞（P = 0.03）、嗜酸性粒细胞（P = 0.025）、肥大细胞（P = 0.03）和CD3（+）淋巴细胞（P = 0.025）增加，但CD4（+）或CD8（+）淋巴细胞计数没有增加。我们还检测到内皮细胞间粘附分子 1 型 (P < 0.05) 和 E-选择素 (P < 0.005) 的增加，但血管细胞粘附分子 1 型表达没有增加，且粘膜下和上皮 LFA(+) 白细胞的相关增加 (P < 0.01)。因此，在过敏性哮喘患者中，局部支气管内过敏原滴注导致气道粘膜炎症细胞浸润增加，这涉及微脉管系统上表达的特异性粘附分子的上调。
We have examined the mucosal changes occurring in bronchial biopsies from six atopic asthmatics 5-6 h after local endobronchial allergen challenge and compared them with biopsies from saline-challenged segments from the same subjects at the same time point. All the subjects developed localized bronchoconstriction in the allergen-challenged segment and had a decrease in forced expiratory volume in 1 s (FEV(1)) (P < 0.01) and a decrease in their methacholine provocative concentration of agonist required to reduce FEV(1) from baseline by 20% (P < 0.05) 24 h postchallenge. At 6 h we observed an increase in neutrophils (P = 0.03), eosinophils (P = 0.025), mast cells (P = 0.03), and CD3(+) lymphocytes (P = 0.025), but not in CD4(+) or CD8(+) lymphocyte counts. We also detected an increase in endothelial intercellular adhesion molecule type 1 (P < 0.05) and E-selectin (P < 0.005), but not vascular cell adhesion molecule type 1 expression with a correlative increase in submucosal and epithelial LFA(+) leucocytes (P < 0.01). Thus, in sensitized asthmatics, local endobronchial allergen instillation leads to an increased inflammatory cell infiltrate of the airway mucosa that involves upregulation of specific adhesion molecules expressed on the microvasculature.