Double knockout of Bax and Bak from kidney proximal tubules reduces unilateral urethral obstruction associated apoptosis and renal interstitial fibrosis.
Double knockout of Bax and Bak from kidney proximal tubules reduces unilateral urethral obstruction associated apoptosis and renal interstitial fibrosis.
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DOI：
10.1038/srep44892
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发表时间：
2017-03-20
影响因子：
4.6
通讯作者：
Dong Z
中科院分区：
文献类型：
--
作者：
Mei S;Li L;Wei Q;Hao J;Su Y;Mei C;Dong Z
间质纤维化是慢性肾脏疾病的常见病理特征，通常与肾组织中的细胞凋亡有关。为了确定相关的细胞凋亡途径及其在肾间质纤维化中的作用，我们建立了一个小鼠模型，其中Bax和巴克，两个关键基因的内在途径的细胞凋亡，特异性地从肾近端小管删除，并使用该模型来检查肾细胞凋亡和间质纤维化后，单侧尿道梗阻（UUO）。结果表明，近端肾小管Bax和巴克基因双敲除可减少肾小管细胞凋亡，抑制UUO肾间质纤维化。结果表明，内源性凋亡途径在肾脏疾病肾小管细胞凋亡和肾间质纤维化中起重要作用。
Interstitial fibrosis, a common pathological feature of chronic kidney diseases, is often associated with apoptosis in renal tissues. To determine the associated apoptotic pathway and its role in renal interstitial fibrosis, we established a mouse model in which Bax and Bak, two critical genes in the intrinsic pathway of apoptosis, were deleted specifically from kidney proximal tubules and used this model to examine renal apoptosis and interstitial fibrosis following unilateral urethral obstruction (UUO). It was shown that double knockout of Bax and Bak from proximal tubules attenuated renal tubular cell apoptosis and suppressed renal interstitial fibrosis in UUO. The results indicate that the intrinsic pathway of apoptosis contributes significantly to the tubular apoptosis and renal interstitial fibrosis in kidney diseases.